We narrowed to 14,369 results for: nts

1. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234372

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-24XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX026

   Plasmid

   #167153

   Purpose

   MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-nnLuz-ocsT
   

   Use

   Luciferase

   Tags

   His6-tag

   Expression

   Plant

   Promoter

   p35s

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TT 3xFLAG-MAVS-Ala271

   Plasmid

   #158631

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Mutation

   Q271A please see depositor comments below

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. plx304-PHB-V5

   Plasmid

   #122230

   Purpose

   Expresses human PHB attached to a V5 tag

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   Prohibitin (PHB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-N-HA-NEK7 K64M

   Plasmid

   #75143

   Purpose

   Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lys 64 mutated to Met; kinase inactive and F236L …

   Promoter

   CMV

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p_sc-eVIP

   Plasmid

   #168174

   Purpose

   Expresses barcoded coding genetic variants in mammalian cells

   Depositor

   James T. Neal, Jesse Boehm, Aviv Regev

   Article

   Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905.

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   EF-1a

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSS02:cyt-Peredox-mCherry_DS

   Plasmid

   #161747

   Purpose

   cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

   Depositor

   Markus Schwarzländer

   Article

   Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

   Insert

   cyt-Peredox-mCherry_DS
   

   Expression

   Plant

   Mutation

   amino acid substitution D115S in first T-Rex doma…

   Promoter

   Ubiquitin 10

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EIF2B3

   Plasmid

   #172072

   Purpose

   In vitro transcription for rescue experiments

   Depositor

   Cheol-Hee Kim

   Article

   Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

   Insert

   EIF2B3 (EIF2B3 Human)
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   July 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid

    #123182

    Purpose

    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites; codon-optimised for plants

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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