We narrowed to 4,337 results for: Pet2

1. 2QO2 (EPHA3)

   Plasmid

   #101271

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHA3 (EPHA3 Human)
   

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. CTL2

   Plasmid

   #92212

   Purpose

   Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

   Depositor

   Douglas Rees

   Article

   Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

   Insert

   CTL2 of PIEZO1
   

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ppj219(JBEI-13475)

   Plasmid

   #89785

   Purpose

   for expression of N-StrepII-3607

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Insert

   3607
   

   Tags

   StrepII

   Expression

   Bacterial

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ppj184 (JBEI-13431)

   Plasmid

   #89784

   Purpose

   for expression of N-His-3607

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Insert

   3607
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ppj187 (JBEI-13437)

   Plasmid

   #89783

   Purpose

   for expression of C-His-3607

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Insert

   3607
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK_proNR2_LGK.9

   Plasmid

   #81162

   Purpose

   Expresses levoglucosan kinase variant LGK.9 with promoter proNR2

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al ACS Synth Biol. 2015 Sep 14.

   Insert

   Levoglucosan kinase
   

   Tags

   His6x

   Expression

   Bacterial

   Mutation

   LGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…

   Promoter

   proNR2

   Available Since

   Sept. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lf-MetQ

   Plasmid

   #78305

   Purpose

   Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

   Depositor

   Douglas Rees

   Article

   Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

   Insert

   Methionine Binding Protein (metQ )
   

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. His10-PS-SNAPf-9R (XSB525)

   Plasmid

   #78513

   Purpose

   Construct for expressing His10 - SNAPf -9R in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   SNAPf
   

   Tags

   His10 followed by a prescission protease claevage…

   Expression

   Bacterial

   Mutation

   9R added to the C-terminal of SNAPf

   Available Since

   June 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK044 (pET-PurQ-wt)

    Plasmid

    #73839

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK598

    Plasmid

    #72523

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK560

    Plasmid

    #71585

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Expression

    Bacterial

    Mutation

    changed arginine-232 to histidine

    Promoter

    T7 promoter

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK504

    Plasmid

    #71584

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-357 to tyrosine

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK612

    Plasmid

    #72533

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Mutation

    residues 1-69 only; C-terminal domain removed and…

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK613

    Plasmid

    #72534

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    residues 1-69 only; C-terminal domain removed and…

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK619

    Plasmid

    #72536

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pfMDH_K381A

    Plasmid

    #67732

    Purpose

    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 381 to alanine

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pfMDH_N300D

    Plasmid

    #67728

    Purpose

    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to aspartate

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pfMDH_R373A

    Plasmid

    #67731

    Purpose

    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed arginine 373 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pfMDH_N300S

    Plasmid

    #67729

    Purpose

    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to serine

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only