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pET-DEST42-ARF4-V5-His6

Plasmid

#67413

Purpose

Expression of hArf4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF5-V5-His6

Plasmid

#67414

Purpose

Expression of hArf5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL1-V5-His6

Plasmid

#67416

Purpose

Expression of hArl1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL2-V5-His6

Plasmid

#67417

Purpose

Expression of hArl2 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL5A-V5-His6

Plasmid

#67420

Purpose

Expression of hArl5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 70 C to T; silent mutation

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4D-V5-His6

Plasmid

#67426

Purpose

Expression of hArl4D with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL14-V5-His6

Plasmid

#67427

Purpose

Expression of hArl14 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 172 C to T; aa L58F

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL11-V5-His6

Plasmid

#67428

Purpose

Expression of hArl11 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 442 T to C; aa C148R

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARFRP1-V5-His6

Plasmid

#67429

Purpose

Expression of hArfrp with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET30-SBL1(160S,HisTagDel)

Plasmid

#66880

Purpose

Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160S)

Tags

none

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160E,HisTagDel)

Plasmid

#66879

Purpose

Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E)

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160S,NoCys)

Plasmid

#66877

Purpose

Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1

Tags

Histag

Expression

Bacterial

Mutation

four (all) cysteines mutated to serine

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160E,NoCys)

Plasmid

#66875

Purpose

Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations

Tags

Histag

Expression

Bacterial

Mutation

S160E; changed 4 (all) cysteines to serine

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(T214V)

Plasmid

#66137

Purpose

Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 G108C/P186C

Plasmid

#66174

Purpose

Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

G108C, P186C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Guinea Pig 1-88)

Plasmid

#62059

Purpose

Expression of Guinea Pig p53 (residues 1-88) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-88

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pET-20b-T7-Ntx-K49

Plasmid

#58715

Purpose

Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Changed Aspartic Acid 49 to Lysine

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET22b-EC12-129/138Avi

Plasmid

#47927

Purpose

Expresses E-cadherin EC12 with C9A, K129C/D138C mutations and avi tag in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Tags

6xHis, Avi Tag, and TEV Cleavage

Expression

Bacterial

Mutation

Mutations C9A, K129C, D138C

Promoter

T7

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y32F Y96F

Plasmid

#47092

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only