Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 17,391 results for: puro

Showing: 9151 - 9200 of 17391 results

  1. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

    Enlarge

  2. p663-UBC-miniTurbo-V5-dsRed_IDG-K

    Plasmid
    #135260
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135260-plasmid-map-sequence-id-311150

    Enlarge

  4. pHAGE-CMV-Flag-hcRed_IDG-K

    Plasmid
    #135274
    Purpose
    Gateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-hcRed
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    135274-plasmid-map-sequence-id-267146

    Enlarge

  5. ELL3 Streptag CtoA

    Plasmid
    #127283
    Purpose
    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHQK
    Promoter
    EF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127283-plasmid-map-sequence-id-250049

    Enlarge

  6. ELL1 Streptag

    Plasmid
    #127278
    Purpose
    Expresses mouse ELL1 with streptactin tag in mammalian cells
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127278-plasmid-map-sequence-id-250019

    Enlarge

  7. pPyCAG-Nanog(Q124A)-IP

    Plasmid
    #62993
    Purpose
    Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q124A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 124 (125 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62993-plasmid-map-sequence-id-107891

    Enlarge

  8. pPyCAG-Nanog(M125A)-IP

    Plasmid
    #62994
    Purpose
    Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog M125A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Methionine 125 (126 AA in Mouse Protein) …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62994-plasmid-map-sequence-id-107893

    Enlarge

  9. pPyCAG-Nanog(Q138A)-IP

    Plasmid
    #62997
    Purpose
    Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q138A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 138 (139 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62997-plasmid-map-sequence-id-104599

    Enlarge

  10. pPyCAG-Nanog(T141A)-IP

    Plasmid
    #62999
    Purpose
    Expresses Mouse Nanog T141A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog T141A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Threonine 141 (142 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62999-plasmid-map-sequence-id-104602

    Enlarge

  11. pPyCAG-Nanog(Q144A)-IP

    Plasmid
    #63000
    Purpose
    Expresses Mouse Nanog Q144A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q144A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 144 (145 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63000-plasmid-map-sequence-id-104604

    Enlarge

  12. pPyCAG-Nanog(N145A)-IP

    Plasmid
    #63001
    Purpose
    Expresses Mouse Nanog N145A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog N145A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Asparagine 145 (146 AA in Mouse Protein) …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63001-plasmid-map-sequence-id-104605

    Enlarge

  13. pPyCAG-Nanog(R147A)-IP

    Plasmid
    #63002
    Purpose
    Expresses Mouse Nanog R147A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog R147A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Arginine 147 (148 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63002-plasmid-map-sequence-id-104608

    Enlarge

  14. pPyCAG-Nanog(K151A)-IP

    Plasmid
    #63004
    Purpose
    Expresses Mouse Nanog K151A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K151A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 151 (152 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63004-plasmid-map-sequence-id-104611

    Enlarge

  15. pPyCAG-Nanog(T100A)-IP

    Plasmid
    #62989
    Purpose
    Expresses Mouse Nanog T100A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog T100A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Threonine 100 (101AA) to Alanine
    Promoter
    CAG
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62989-plasmid-map-sequence-id-104167

    Enlarge

  16. pPyCAG-Nanog(F102A)-IP

    Plasmid
    #62990
    Purpose
    Expresses Mouse Nanog F102A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog F102A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 102 (103AA in Mouse Protein…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62990-plasmid-map-sequence-id-107872

    Enlarge

  18. pX459_CDC45_sgRNA

    Plasmid
    #232732
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232732-plasmid-map-sequence-id-465449

    Enlarge

  19. pLKO.1C

    Plasmid
    #139470
    Purpose
    Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.
    Depositor
    David Cobrinik
    Article
    Lee et al Biotechniques. 2020 Jun;68(6):349-352. d
    Insert
    stuffer
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    139470-plasmid-map-sequence-id-337626

    Enlarge

  21. pLIX-hN1ICD

    Plasmid
    #91897
    Purpose
    Doxycycline inducible expression of human Notch1 ICD
    Depositor
    Julien Sage
    Insert
    Notch1 intracellular domain (NOTCH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codons 1770 to 2555 of human NOTCH1
    Promoter
    TRE promoter, Tet ON
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91897-plasmid-map-sequence-id-180657

    Enlarge

  22. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid
    #155045
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Glutaredoxin-1 (GLRX Human)
    Use
    Lentiviral
    Tags
    roGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155045-plasmid-map-sequence-id-310403

    Enlarge

  32. pMEV4

    Plasmid
    #110539
    Purpose
    The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.
    Depositor
    William B. Whitman
    Article
    Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    110539-plasmid-map-sequence-id-211531

    Enlarge

  33. pLenti-SFNp-mRFP1

    Plasmid
    #238473
    Purpose
    Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone
    Depositor
    Christopher Nelson
    Insert
    SFNp-mRFP1
    Use
    Tags
    Expression
    Mutation
    Promoter
    SFNp
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238473-plasmid-map-sequence-id-472675

    Enlarge

  34. pTRIPZ (M)-HT-Cbx4

    Plasmid
    #82513
    Purpose
    Lentivirus vector. Expresses Halotag-Cbx4 fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 4 (Cbx4 Mouse)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82513-plasmid-map-sequence-id-164664

    Enlarge

  36. pLJM1-4EBP1-T37A/T46A/Y54A/L59A

    Plasmid
    #136421
    Purpose
    Stable overexpression of elF4EBP1 T37A/T46A/Y54A/L59A
    Depositor
    Gabriel Leprivier
    Insert
    eIF4EBP1 (EIF4EBP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T37A/T46A/Y54A/L59A
    Promoter
    CMV
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    136421-plasmid-map-sequence-id-269592

    Enlarge

  43. pJPF0117

    Plasmid
    #130267
    Purpose
    Lentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFP
    Depositor
    Hana El-Samad
    Article
    Fonseca et al bioRxiv 2020, 747030
    Insert
    bPAC
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    130267-plasmid-map-sequence-id-254858

    Enlarge

  48. pLVX-EGFP-C3-Cep123

    Plasmid
    #73330
    Purpose
    Lentiviral vector encoding Cep123 isoform 1 with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    Cep123 (CEP89 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73330-plasmid-map-sequence-id-139047

    Enlarge

  49. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

    Enlarge

Showing: 9151 - 9200 of 17391 results