We narrowed to 3,744 results for: AES

1. pAS1_4x7SKPylT_EF1_Ub-*SMIM38

   Plasmid

   #162808

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled SMIM38

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)SMIM38
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   SMIM38 (Met1, Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4x7SKPylT_EF1_Ub-*PIGBOS

   Plasmid

   #162809

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)PIGBOS
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   PIGBOS (Met1, Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1-PPARA

   Plasmid

   #169019

   Purpose

   Expression of PPARA driven by CMV promoter

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   human ppara (PPARA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_gRNA pAS dual_hspCas9

   Plasmid

   #193312

   Purpose

   Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459_gRNA pAS single_hspCas9

   Plasmid

   #193311

   Purpose

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

   Plasmid

   #162804

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI_IRES_Blasticidin
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC_h7SK Mma PylT (CUA)

   Plasmid

   #154782

   Purpose

   plasmid with single copy of Mma PylT (CUA anticodon)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   U25C

   Promoter

   h7SK

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC_h7SK Mma PylT (UUA)

   Plasmid

   #154783

   Purpose

   plasmid with single copy of Mma PylT (UUA anticodon)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   Mma PylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   U25C and UUA anticodon

   Promoter

   h7SK

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1_4x7SKPylT_EF1_Ub-BsmBI

   Plasmid

   #162801

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

    Plasmid

    #162811

    Purpose

    Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_(C1A)-Intein_DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Intein(Cys1,Ala)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

    Plasmid

    #162815

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_M
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_M(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

    Plasmid

    #162816

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_9b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_9b(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

    Plasmid

    #162817

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF6
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF6(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

    Plasmid

    #162818

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF7b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF7b(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

    Plasmid

    #162819

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF10
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF10(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

    Plasmid

    #162820

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF9c/14
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF9c/14(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

    Plasmid

    #162821

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b_alt
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b_alt(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

    Plasmid

    #162822

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b(CoV1h)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b(CoV1h)(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC_h7SK M15 (CUA)

    Plasmid

    #154784

    Purpose

    plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G10A, G14U, G16U, C20U, C52A

    Promoter

    h7SK

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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