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pDM097

Plasmid

#216819

Purpose

Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mStayGold

Tags

FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.Mark2-3myc

Plasmid

#224416

Purpose

Mammalian expression of MARK2

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

MARK2 (MARK2 Human)

Tags

3myc

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP(FLAG/HA-NSP1-WT)

Plasmid

#188781

Purpose

Expresses FLAG/HA-NSP1 in mammalian cells (SARS-CoV-2 NSP1)

Depositor

Markus Hafner

Article

Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

Insert

FLAG/HA-NSP1 (SARS-CoV-2)

Use

Frt site to insert into hek293 t-rex genome

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-PILATeS-RGD (200nM)

Plasmid

#216716

Purpose

Expresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-PILATeS-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-PILATeS-RGD (700pM)

Plasmid

#216714

Purpose

Expresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-PILATeS-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Nm2Cas9-BlastR

Plasmid

#220980

Purpose

Expresses human codon-optimized Nm2Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Nm2Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-St1Cas9-BlastR

Plasmid

#220976

Purpose

Expresses human codon-optimized St1Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

St1Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SlugCas9-HF-BlastR

Plasmid

#220973

Purpose

Expresses human codon-optimized SlugCas9-HF

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SlugCas9-HF

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SauriCas9-KKH-BlastR

Plasmid

#220972

Purpose

Expresses human codon-optimized SauriCas9-KKH

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9-KKH

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Sa-SlugCas9-BlastR

Plasmid

#220968

Purpose

Expresses human codon-optimized Sa-SlugCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Sa-SlugCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DmPGC

Plasmid

#220309

Purpose

red-light-activated guanylyl cyclase DmPGC

Depositor

Andreas Moeglich

Article

Xu et al J Mol Biol. 2024 Mar 1;436(5):168257. do

Insert

DmPGC

Expression

Mammalian

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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RT-5

Plasmid

#220442

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (puf-3 Nematode)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.Z-GS3

Plasmid

#204758

Purpose

Expression of Cas9 and human H2A.Z

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AZ1 (H2AZ1 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.1-GS3

Plasmid

#204754

Purpose

Expression of Cas9 and human H2A.1

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A.1 (H2AC17 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.2-GS3

Plasmid

#204755

Purpose

Expression of Cas9 and human H2A.2

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A.2 (H2AC18 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GCaMP6f-WPRE

Plasmid

#216275

Purpose

AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

human Synapsin 1 (hSyn)

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4693

Plasmid

#200784

Purpose

Ptwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

no

Tags

GFP

Expression

Worm

Promoter

Ptwk-40s

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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2x3APWWP-IFP2.0C

Plasmid

#215752

Purpose

Expresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-HTR2A-V2R-NTEV-tcs-GV-2xHA

Plasmid

#214615

Purpose

HTR2A receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

HTR2A-NTEV (HTR2A Human)

Tags

TEV-Gal4-VP16-2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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