We narrowed to 4,135 results for: pCMV mammalian

1. nCre-ST-NLS-P2A-NLS-pSC-cCre

   Plasmid

   #207638

   Purpose

   A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
   

   Expression

   Mammalian

   Mutation

   Amber stop codon at SpyCatcher’s critical lysine

   Promoter

   CMV

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA_B2AR-no-pep

   Plasmid

   #47439

   Purpose

   control FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

3. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPKm-105

   Plasmid

   #104853

   Purpose

   pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   PhyB NT GBD
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA_B2AR-q-pep

   Plasmid

   #47437

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. cmv hTid Short H121Q

   Plasmid

   #13710

   Depositor

   Karl Munger

   Article

   Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

   Insert

   hTid Short H121Q (DNAJA3 Human, hTid Short H121Q)
   

   Expression

   Mammalian

   Mutation

   H121Q

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

7. cmv hTid Long H121Q

   Plasmid

   #13708

   Depositor

   Karl Munger

   Article

   Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

   Insert

   hTid Long H121Q (DNAJA3 Human)
   

   Expression

   Mammalian

   Mutation

   H121Q

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

8. p5M-PR-A

   Plasmid

   #89124

   Purpose

   mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   human progesterone receptor-A (PGR Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. miABE

   Plasmid

   #205412

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
   

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-jGCaMP7s

    Plasmid

    #104463

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7s
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALED_Right-mouse-ATP6-1397C-AD

    Plasmid

    #212954

    Purpose

    Expresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-CMV-jGCaMP7b

    Plasmid

    #104484

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7b
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Td-CGBE

    Plasmid

    #196599

    Purpose

    Mammalian expression of Td-CGBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N46L); S. pyogenes Cas9 (D10A)

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-SasgRNA scaffold-CMV-nSaCas9

    Plasmid

    #171695

    Purpose

    Expresses nSaCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    sgRNA scaffold-nSaCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. A&C-BEmax

    Plasmid

    #157947

    Purpose

    Mammalian expression of A&C-BEmax

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    A&C-BEmax
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-jGCaMP7c variant 1513

    Plasmid

    #105320

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7c variant 1513
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TALED_Right-mouse-ATP6-1397C-AD(V28R)

    Plasmid

    #212955

    Purpose

    Expresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    Right-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PEA1-Puro-HEK3 CTT ins

    Plasmid

    #171996

    Purpose

    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A

    Plasmid

    #212949

    Purpose

    Expresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNK3203

    Plasmid

    #219749

    Purpose

    DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - mcitHispS - tSV40
    

    Expression

    Mammalian

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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