We narrowed to 14,886 results for: pET

1. pEW3: pET-32a Dot1L(2-416)

   Plasmid

   #124098

   Purpose

   Dot1L(2-416) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-416) (DOT1L Human)
   

   Use

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b/GgVcl 884-1066-ECFP

   Plasmid

   #46182

   Depositor

   Susan Craig

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Use

   Tags

   ECFP and His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (tail domain)

   Promoter

   T7

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-His10-SHP1_PTPase (243-541aa)

   Plasmid

   #180812

   Purpose

   Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   SHP1_PTPase (243-541aa) (PTPN6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOXB8 (murine) HIS-tag pET

   Plasmid

   #8540

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB8 (Hoxb8 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   starts at amino acid #2 (Ser)

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pETNKI-his-SUMO-RNF168ring (1-189)

   Plasmid

   #63570

   Purpose

   Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   6xHis and SUMO3

   Expression

   Bacterial

   Mutation

   Ring domain only (residues 1-189)

   Promoter

   T7

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET-His6-Sumo-MBP-CfaN

   Plasmid

   #126016

   Purpose

   Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

   Depositor

   Thomas Muir

   Insert

   His5-Sumo-MBP-CfaN
   

   Use

   Tags

   5xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a-His6-SUMO-VAMP3

   Plasmid

   #135552

   Purpose

   Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP3
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-Streptavidin-Dead Aspartate Loop

   Plasmid

   #46368

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Dead Aspartate Loop
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET29b-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175783

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-45b-RUNX2(WT)-IDR-mCherry

    Plasmid

    #145286

    Purpose

    Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(WT)-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Use

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET empty polycistronic destination vector (2Z)

    Plasmid

    #29776

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-Vps4(101-437)-Hcp1

    Plasmid

    #87737

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HOXA7 (murine) His-tag pET

    Plasmid

    #8491

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA7 (Hoxa7 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG; makes protein by …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pET15b/GgVcl 1-1066 (full length)

    Plasmid

    #46171

    Depositor

    Susan Craig

    Article

    Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

    Insert

    Vcl (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-45b-HOXD13-IDR-mCherry

    Plasmid

    #145280

    Purpose

    Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-ybbR-HIS-sfGFP-DocI

    Plasmid

    #58708

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    sfGFP-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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