We narrowed to 26,449 results for: Vit;

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psiCHECK-1-BRAF_WT-SM

Plasmid

#215917

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_ WT (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut1-SM

Plasmid

#215918

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut2-SM

Plasmid

#215919

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT&Mut-CM

Plasmid

#215920

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT-CM

Plasmid

#215921

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ WT (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut1-CM

Plasmid

#215922

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut1 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut2-CM

Plasmid

#215923

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1L

Plasmid

#215043

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

Left portion of TWIST1 bHLH loop (aa137-139, TLP)…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1R

Plasmid

#215044

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

Right portion of TWIST1 bHLH loop (aa139-141, PSD…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA EIVK (E1658K) pcDNA3

Plasmid

#206066

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

E1658K

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pT22UbhaEng2aE4PCh(#271)

Plasmid

#184085

Purpose

cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

3HA-ZfEng2a-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

3xHA

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22UbhaEng2bE4PCh(#272)

Plasmid

#184086

Purpose

cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

3HA-ZfEng2b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

3xHA

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22gCfpUbm5En2E4PCh(#251)

Plasmid

#184083

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm5Eng2bE4PCh(#284)

Plasmid

#184088

Purpose

cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22UbHEn2E4PCh(#262)

Plasmid

#184084

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

3HA-chkEn2-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

3xHA

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm5Eng2aE4PCh(#283)

Plasmid

#184087

Purpose

cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2a-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a-KRAB_crRNA Td2

Plasmid

#176261

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

Kruppel associated box domain and luciferase

Mutation

D917A and E1006A mutations to inactivate the endo…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-delta458-500

Plasmid

#97074

Purpose

Expresses mutant CARD14/CARMA2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 458-500

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00021

Plasmid

#34183

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG14548 (E(spl)mbeta-HLH Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-iGluSnFR4s-NGR-WPRE

Plasmid

#234451

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al bioRxiv 2025.03.20.643984

Insert

iGluSnFR4s-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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