We narrowed to 7,241 results for: CIT

1. pUPD2 sgRNA sCRNA 2.0 (GB2461)

   Plasmid

   #160593

   Purpose

   Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA sCRNA 2.0
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

   Plasmid

   #160625

   Purpose

   GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   U6-26-1gRNA-DFR 2.1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

   Plasmid

   #160570

   Purpose

   A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA:scaffold tetraloop MS2 aptamer SAM
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28A-p53 (1-73) (PPP)

   Plasmid

   #62067

   Purpose

   Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains TAD residues 1-73; P12A, P13A, P27A

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PX458-3xHA-FnCas9

   Plasmid

   #130969

   Purpose

   3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   FnCas9
   

   Use

   CRISPR

   Tags

   3xHA-NLS and NLS-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EWSR1::WT1(-KTS)

   Plasmid

   #220565

   Purpose

   Lentiviral expression of human EWSR1::WT1(-KTS)

   Depositor

   Sean Lee

   Article

   Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

   Insert

   EWSR1::WT1(-KTS) (EWSR1, WT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EWSR1::WT1(+KTS)

   Plasmid

   #220566

   Purpose

   Lentiviral expression of human EWSR1::WT1(+KTS)

   Depositor

   Sean Lee

   Article

   Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

   Insert

   EWSR1::WT1(+KTS) (WT1, EWSR1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-TetO-hNGN2-eGFP-Puro

   Plasmid

   #79823

   Purpose

   Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

   Depositor

   Kristen Brennand

   Article

   Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

   Insert

   hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TetO

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

   Plasmid

   #170226

   Purpose

   Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   IKBKB (IKBKB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   S177E, S181E

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid

    #170236

    Purpose

    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S176E, S180E

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

    Plasmid

    #170237

    Purpose

    Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV phSyn1(S)-DreO-bGHpA

    Plasmid

    #50363

    Purpose

    Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV Retrograde and AAV5

    Insert

    DreO recombinase
    

    Use

    AAV

    Tags

    none

    Expression

    Mutation

    Sequence optimized for expression in mammalian ce…

    Promoter

    hSyn1

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG303-Gal-PA50

    Plasmid

    #84906

    Purpose

    induce expression of dipeptide repeat PA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    PA50 (C9orf72 Synthetic)
    

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG303-Gal-GA50

    Plasmid

    #84907

    Purpose

    induce expression of dipeptide repeat GA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    GA50 (C9orf72 Synthetic)
    

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMC00005

    Plasmid

    #166722

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMC00006

    Plasmid

    #166723

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER20-Tau

    Plasmid

    #92201

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for Tau

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-2dV-Camui (wt)

    Plasmid

    #220366

    Purpose

    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    

    Use

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF6-Acly

    Plasmid

    #70765

    Purpose

    Expresses mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Use

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Slc17a7-IRES2-Cre targeting vector

    Plasmid

    #61574

    Purpose

    Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Slc17a7-IRES2-Cre
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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