We narrowed to 24,507 results for: Spr;

1. pU6-sgGAL4-4

   Plasmid

   #46916

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgGAL4-4
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAE1 G7.3 gRNA

   Plasmid

   #90776

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NAE1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NAE1 (Guide Designation G7.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

   Plasmid

   #109311

   Purpose

   Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

   Depositor

   William Lagor

   Article

   Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

   Insert

   Ldlr gRNA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_mCherry_CD19.4

   Plasmid

   #140101

   Purpose

   CRISPR-Cas9 Knock down

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PLK1 G5.1 gRNA

   Plasmid

   #90834

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PLK1 (Guide Designation G5.1)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIK208

   Plasmid

   #65630

   Purpose

   sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_EE-nCas9

   Plasmid

   #163541

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   EE-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

   Plasmid

   #179054

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-TUB1
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

   Plasmid

   #105040

   Purpose

   Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Tsc2 (Tsc2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179067

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-ERG6-Link-Scarlet(rfp)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

    Plasmid

    #179066

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-ATG8
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179060

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PIL1-Link-Scarlet(rfp)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. A3B-Cas9n-UGI-NLS

    Plasmid

    #198889

    Purpose

    Express full-length A3B BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_KDM6A_2

    Plasmid

    #86308

    Purpose

    Encodes gRNA for 3' target of human KDM6A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM6A (KDM6A Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-dAaCas12b-Act3.0

    Plasmid

    #158413

    Purpose

    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1068

    Plasmid

    #205962

    Purpose

    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH proteins and CRISPR array
    

    Expression

    Bacterial

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP2372

    Plasmid

    #73196

    Purpose

    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, and Q926A in SpCas9

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PSMD14 E6.4 gRNA

    Plasmid

    #90859

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PSMD14

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PSMD14 (Guide Designation E6.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTox-EGFPsite1_NGG-PAM (BPK740)

    Plasmid

    #181746

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
    

    Use

    Selection plasmid

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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