We narrowed to 11,764 results for: nsf

1. pRGEB32-CrActin-UBI

   Plasmid

   #226709

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrActin
   

   Use

   CRISPR

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRGEB32-CrU6-2-UBI

   Plasmid

   #226707

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrU6-2
   

   Use

   CRISPR

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKG-GW1-SRT1

   Plasmid

   #203171

   Purpose

   Yeast expression vector for yeast SRT1

   Depositor

   William Sessa

   Article

   Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

   Insert

   SRT1 (SRT1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLINE1ΔORF2-scFvFc

   Plasmid

   #206021

   Purpose

   The ORF2 sequence was removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
   

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLINE1ΔORF1-2-scFvFc

   Plasmid

   #206022

   Purpose

   The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR4 genomic A. thaliana PLDGAMMA3

   Plasmid

   #209188

   Purpose

   pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   AT4G11840 (PLDGAMMA3 Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Promoter

   none

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHD22Y/PfPRMT5 deletion

   Plasmid

   #203161

   Purpose

   for disruption of PfPRMT5 gene

   Depositor

   Liwang Cui

   Article

   Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

   Insert

   PfPRMT5 fragment
   

   Tags

   3 myc tags and 3' UTR of P. berghei dihydrof…

   Expression

   Mammalian

   Promoter

   No

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKG-GW2-NUS1

    Plasmid

    #203169

    Purpose

    Yeast expression vector for yNUS1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKG-GW2

    Plasmid

    #203164

    Purpose

    Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using methionine selection

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL0_CE-OriT

    Plasmid

    #202150

    Purpose

    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Origin of transfer
    

    Use

    Synthetic Biology

    Mutation

    OriT sequence mutated to remove sapI sites domest…

    Available Since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pKG-GW1-RER2

    Plasmid

    #203170

    Purpose

    Yeast expression vector for yeast RER2

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    RER2 (RER2 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-FRISZ-GPI

    Plasmid

    #177264

    Purpose

    AAV transfer plasmid for cell-surface-localized FRISZ (via CD59 GPI) under hSyn promoter

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    CD59 leader-FRISZ-GPI
    

    Use

    AAV

    Tags

    CD59 GPI and CD59 leader sequence

    Promoter

    hSyn

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNHT7::UMSBP::sNluc2_ORF1

    Plasmid

    #186765

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNHT7::ENO::sNluc2

    Plasmid

    #186770

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNHT7::RPL15::sNluc0

    Plasmid

    #186771

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNHT7::RPL15::hNluc

    Plasmid

    #186772

    Purpose

    An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNHT7::YB1::hNluc

    Plasmid

    #186774

    Purpose

    An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAPDH::sNluc1

    Plasmid

    #187221

    Purpose

    A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Overexpression

    Promoter

    GAPDH

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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