We narrowed to 5,331 results for: pcdna3.1

1. Opto-beta2AR

   Plasmid

   #106063

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-beta2AR
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. VfAU1-LOV_P2A_casp9

   Plasmid

   #131224

   Purpose

   Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   VfAU1-LOV-HA_P2A_myc-casp9
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Venus-155-239-Gbeta1

   Plasmid

   #234751

   Purpose

   Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

   Depositor

   Nevin Lambert

   Article

   Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

   Insert

   GNB1 (GNB1 Human)
   

   Tags

   VN155

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. REEP5-mEmerald

   Plasmid

   #186964

   Purpose

   Express REEP5/DP1 with C-terminus mEmerald

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Reep5 (Reep5 Mouse)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Opto-casp9_AtCRY2-PHR-casp9

   Plasmid

   #131221

   Purpose

   Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   Opto-casp9 (AtCRY2-PHR-casp9)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 MEKK1 KD

   Plasmid

   #12180

   Depositor

   Gary Johnson

   Article

   Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

   Insert

   MEKK1 (Map3k1 Mouse)
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Kinase dead: K1253M; V1467L

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

7. SP-GFP-CD274-UTR

   Plasmid

   #121142

   Purpose

   Mammalian expression of GFP tagged CD274 with its own 3'UTR

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   CD274 with its own 3'UTR (CD274 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. WN10151

   Plasmid

   #80441

   Purpose

   Expresses human codon-optimized dead (832A) LbCpf1

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Insert

   dead (D832A) LbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Arl6ip1-mEmerald

   Plasmid

   #186965

   Purpose

   Express Arl6ip1 with C-terminus mEmerald

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   ARL6IP1 (ARL6IP1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Actin Binding Domain Tension Sensor

    Plasmid

    #215368

    Purpose

    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tension Sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Actin Binding Domain Tensionless Control

    Plasmid

    #215371

    Purpose

    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tensionless Control
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid

    #169552

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-NanoLuc Luciferase - mTagBFP fusion
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Opto-alpha1AR

    Plasmid

    #106064

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-alpha1AR
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-dCas9-trCIB1

    Plasmid

    #64119

    Purpose

    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    dCas9-trCIB1 fusion
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    dCas9 (D10A H840A, catalytically inactive), trCIB…

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-HCMVgH (TB40/E)

    Plasmid

    #136446

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-FLAG-Bbs4

    Plasmid

    #128868

    Purpose

    For mammalian expression of mouse Bbs4

    Depositor

    Aimin Liu

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

    Insert

    Bbs4 (Bbs4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FL-mCherry

    Plasmid

    #78862

    Purpose

    DULIP negative control (prey) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Smac(1-60)mCherry

    Plasmid

    #40880

    Depositor

    Douglas Green

    Article

    Tait et al Dev Cell. 2010 May 18;18(5):802-13.

    Insert

    Smac(1-60)mCherry (DIABLO Human)
    

    Tags

    mCherry

    Promoter

    CMV

    Available Since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

    Plasmid

    #169533

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge