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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,636 results for: t7

Showing: 3701 - 3720 of 10636 results

  1. pJK325

    Plasmid
    #71556
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71556-plasmid-map-sequence-id-131415

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  2. pJK345

    Plasmid
    #71559
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 and alanine-368
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71559-plasmid-map-sequence-id-134626

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  3. pJK344

    Plasmid
    #71558
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 (same as deleting alanine-368)
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71558-plasmid-map-sequence-id-134625

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  4. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

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  5. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

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  7. pST50Trc4-HPCNDHFR

    Plasmid
    #63998
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63998-plasmid-map-sequence-id-177404

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  8. pST50Trc4-CBPNDHFR

    Plasmid
    #63995
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63995-plasmid-map-sequence-id-177401

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  9. pST50Trc4-HPCDHFR

    Plasmid
    #63993
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63993-plasmid-map-sequence-id-177399

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  10. pST50Trc3-DHFRCYD

    Plasmid
    #63988
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63988-plasmid-map-sequence-id-177394

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  11. pST50Trc3-HPCDHFR

    Plasmid
    #63975
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63975-plasmid-map-sequence-id-177381

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  12. pST50Trc3-HPCHISNDHFR

    Plasmid
    #63984
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63984-plasmid-map-sequence-id-177534

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  13. pST50Trc2-HPCNDHFR

    Plasmid
    #63962
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63962-plasmid-map-sequence-id-177368

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  14. pST50Trc3-FLAGDHFR

    Plasmid
    #63974
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    FLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63974-plasmid-map-sequence-id-177380

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  15. pST50Trc1-HPCHISNDHFR

    Plasmid
    #63948
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63948-plasmid-map-sequence-id-177464

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  16. pST50Trc1-CBPHISNDHFR

    Plasmid
    #63945
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Calmodulin Binding Peptide (CBP); TEV…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63945-plasmid-map-sequence-id-177351

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  17. pST50Trc4-STRDHFR

    Plasmid
    #63991
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63991-plasmid-map-sequence-id-177397

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  18. pST50Trc3-STRHISNDHFR

    Plasmid
    #63982
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63982-plasmid-map-sequence-id-177388

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  19. pST50Trc3-HPCNDHFR

    Plasmid
    #63980
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63980-plasmid-map-sequence-id-177386

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  20. pST50Trc3-CBPNDHFR

    Plasmid
    #63977
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63977-plasmid-map-sequence-id-177383

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Showing: 3701 - 3720 of 10636 results