We narrowed to 15,826 results for: grna

1. Donor-attp-u6-blank-sgrna-backbone

   Plasmid

   #234076

   Purpose

   Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Insert

   sgRNA-Blank
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

   Plasmid

   #216166

   Purpose

   Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   phU6, ph7SK, phH1, pmU6

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY2259 hNOLC1 atgRNA Paired Guide 1

   Plasmid

   #220989

   Purpose

   hNOLC1 atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY2260 hNOLC1 atgRNA Paired Guide 2

   Plasmid

   #220990

   Purpose

   hNOLC1 atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY2261 hACTB atgRNA Paired Guide 1

   Plasmid

   #220991

   Purpose

   hACTB atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDY2262 hACTB atgRNA Paired Guide 2

   Plasmid

   #220992

   Purpose

   hACTB atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-MM002-ZFP143-C-terminal-sgRNA

   Plasmid

   #212706

   Purpose

   Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

   Depositor

   Elzo de Wit

   Insert

   ZFP143 C-terminal sgRNA (Zfp143 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223323

   Purpose

   Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Insert

   SpCas9 and sgRNA-T2 (cas9 )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC130: pAAV.CMV-CasRx-VEGFA sgRNA

   Plasmid

   #203443

   Purpose

   Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   RfxCas13d
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

    Plasmid

    #203447

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

    Plasmid

    #203451

    Purpose

    Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimised using GenScript tool

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

    Plasmid

    #227800

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA 2 (Vegfa Synthetic)
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

    Plasmid

    #227801

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA 3 (Vegfa Synthetic)
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

    Plasmid

    #209922

    Purpose

    A knock-out vector for the dog CDH1

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    A gRNA targeting the dog CDH1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HEK4-53bp-tag-insertion-pegRNA

    Plasmid

    #227673

    Purpose

    PegRNA for 53 bp insertion

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    SpCas9 sgRNA scaffold (EPHA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

    Plasmid

    #206178

    Purpose

    Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

    Depositor

    Yuxuan Guo

    Article

    Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    cTnT

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-U6.3-sgRNA-empty-GFP

    Plasmid

    #221843

    Purpose

    empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Insert

    EGFP
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGC

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-U6.3-sgRNA-DNM1L -GFP

    Plasmid

    #221845

    Purpose

    Tol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGC and U6.3 chick

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tol2-U6.3-sgRNA-MFN1-GFP

    Plasmid

    #221846

    Purpose

    Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGC and U6.3 chick

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SL3-MS2-gRNA GG backbone (MOBE3-4)

    Plasmid

    #219949

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-SL3 (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX552-EF1a-DIO-mCherry_Dicer gRNA

    Plasmid

    #215279

    Purpose

    Expresses Dicer gRNA in a Cre dependent mcherry vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Dicer control gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX552-EF1a-DIO-mCherry_DicerTTT gRNA

    Plasmid

    #215280

    Purpose

    Expresses Dicer Control gRNA in a Cre dependent mcherry vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Dicer control gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

    Plasmid

    #215281

    Purpose

    Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

    Plasmid

    #215282

    Purpose

    Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 control gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

    Plasmid

    #220494

    Purpose

    To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting LIG4 exon 3 (LIG4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-GG-hUbC-EBPF2-gRNA-FAM13A

    Plasmid

    #185550

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting FAM13A

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    FAM13A gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-N1303K-ngRNA+14_EF1a-puroR

    Plasmid

    #207359

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR +14 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-L227R-ngRNA+15_EF1a-puroR

    Plasmid

    #207360

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    L227R-CFTR +15 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCF821-recipient_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211651

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge