We narrowed to 3,823 results for: Gateway entry

Showing: 3721 - 3740 of 3823 results

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pENTR221-APP751

Plasmid

#193772

Purpose

Entry vector for human APP isoform having 751 amino acid residues

Depositor

Wade Harper

Publication

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

APP (APP Human)

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

Plasmid

#121835

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-Luc-A

Plasmid

#25756

Purpose

Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pYZ714

Plasmid

#132955

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Publication

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Promoter

tight TRE

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiR_Gb2-K

Plasmid

#25766

Purpose

Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Gateway Cloning

Tags

GFP

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE4

Plasmid

#195370

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

Depositor

Yiping Qi

Publication

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GGGGS)x6-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

Tags

5' and 3' NLS

Expression

Plant

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-pax3a-5.4k

Plasmid

#164966

Purpose

zebrafish pax3a -5.4k promoter

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

pax3a (pax3a Zebrafish)

Use

Gateway 5 end entry vector

Mutation

promoter -5.4k

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pZA13

Plasmid

#158491

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

no STOP codon

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, STOP codon

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Publication

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Gateway Cloning

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-SLC7A11 sh926R

Plasmid

#118701

Purpose

entry clone for SLC7A11 insensitive to hairpin TRCN0000288926

Depositor

William Kaelin

Publication

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

SLC7A11 (SLC7A11 Human)

Expression

Mammalian

Mutation

wt , SLC7A11 sh926R

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE1

Plasmid

#195367

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

Depositor

Yiping Qi

Publication

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

Tags

3xflag

Expression

Plant

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLEG(R1-R4) #BGV057

Plasmid

#48957

Purpose

Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

Depositor

David Dankort

Publication

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pENTR1A mKate2-hu_ncOGT D554N

Plasmid

#154283

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Publication

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

D554N mutation (Ser/Thr Glycosylation Impaired)

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBEG R3-miRNA(ccdB)-L4 #BGV228

Plasmid

#48953

Purpose

To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

Depositor

David Dankort

Publication

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway Cloning

Promoter

na

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pENTR2b-mVenus

Plasmid

#154898

Purpose

mVenus entry vector

Depositor

Johanna Ivaska

Publication

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Type

Empty backbone

Use

Gateway Cloning

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-mDIXDC1 WT

Plasmid

#61216

Purpose

Entry clone encoding long isoform of mouse DIXDC1

Depositor

Reuben Shaw

Publication

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

DIXDC1 (Dixdc1 Mouse)

Use

Donr clone (gateway)

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221 Cas9-HMGB1

Plasmid

#183198

Purpose

Entry clone

Depositor

Emma Haapaniemi

Publication

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-HMGB1

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Publication

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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