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pEBG MKK3b (Glu)

Plasmid

#50451

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Use

Tags

Gst

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

SV40

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
1287 p3E-mut let-7 etv2 3’ UTR

Plasmid

#49011

Purpose

Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Tags

Expression

Mutation

Mutated the Let7 miRNA binding sites

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only
pFM038

Plasmid

#40141

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R183-5A + 7SA; base pairs 6-523 of par-2 were re…

Promoter

pie-1

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM101

Plasmid

#40147

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 wild-type RNAi resistant (par-2 Nematode)

Use

Tags

GST and eGFP

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create …

Promoter

tac

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFM102

Plasmid

#40148

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)

Use

Tags

GST and eGFP

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded t…

Promoter

tac

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFM041

Plasmid

#40144

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (S241A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM039

Plasmid

#40142

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R183-5A + S241A; base pairs 6-523 of par-2 were …

Promoter

pie-1

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM036

Plasmid

#40139

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (K162A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM052

Plasmid

#40146

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (C56S) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

pie-1

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pFM037

Plasmid

#40140

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R163A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pRLCon1288-1666

Plasmid

#31448

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon183-221_850-1207

Plasmid

#31449

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon183-221mut2_850-1207

Plasmid

#31450

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mut2 in balancer

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon1-449_850-1207

Plasmid

#31451

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAL119-TK

Plasmid

#21911

Depositor

Maria Castro

Article

Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMSCV-Flag-hOct4

Plasmid

#20072

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hOct4 (POU5F1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_cAMPFIRE-L-R279E

Plasmid

#182286

Purpose

Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_cAMPFIRE-H

Plasmid

#182285

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge