We narrowed to 6,084 results for: nls

1. pLSLZ

   Plasmid

   #51495

   Purpose

   pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   3kb filler sequence
   

   Use

   Plant t-dna plasmid

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   virion-sense LIR and 2x35S

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBALU18

   Plasmid

   #69334

   Purpose

   Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2ndmChO intron; use C-term after STOP

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   rSL2-NLS-mChOint-FgF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pIVT-evoBxb1

   Plasmid

   #232747

   Purpose

   IVT template for making modRNA encoding evoBxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   V74A

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVT-Bxb1-I87L

   Plasmid

   #232749

   Purpose

   IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   I87L

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL390

   Plasmid

   #219500

   Purpose

   Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
   

   Use

   Tags

   SV40-NLS

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-GAL10

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDL25_Cas9-His Δcys E1207C

   Plasmid

   #206286

   Purpose

   Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1207C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL23_Cas9-His Δcys E945C

   Plasmid

   #206284

   Purpose

   Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E945C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDL24_Cas9-His Δcys E1026C

   Plasmid

   #206285

   Purpose

   Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1026C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL22_Cas9-His Δcys E532C

   Plasmid

   #206283

   Purpose

   Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-REPORT(EF1a no GFP)

    Plasmid

    #172339

    Purpose

    mnucTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-REPORT(EF1a)TetR

    Plasmid

    #172352

    Purpose

    Tet Repressor reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    TetR
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENmRFPCNAL2 pc1054

    Plasmid

    #167567

    Purpose

    The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

    Depositor

    Cristina Cardoso

    Article

    Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAL1-VP1 (LEU2)

    Plasmid

    #182480

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT15065-HF-CBE

    Plasmid

    #163010

    Purpose

    plasmid expressing HF-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAT15066-Hypa-CBE

    Plasmid

    #163011

    Purpose

    plasmid expressing Hypa-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT15067-HypaR661A-CBE

    Plasmid

    #163012

    Purpose

    plasmid expressing HypaR661A-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    R661A

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAT15069-HeF-CBE

    Plasmid

    #163014

    Purpose

    plasmid expressing HeF-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HeF-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT15000-BE4max

    Plasmid

    #163015

    Purpose

    plasmid expressing BE4max

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT9995-evoABE

    Plasmid

    #163003

    Purpose

    plasmid expressing evoABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evoABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAT9784-xABE

    Plasmid

    #163005

    Purpose

    plasmid expressing xABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    xABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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