We narrowed to 2,326 results for: PUC19

1. pGGB-V5-TurboID-T2A-C

   Plasmid

   #218896

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITESceIcmd

   Plasmid

   #218288

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253, -1)-tSynth3

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

   Plasmid

   #173208

   Purpose

   HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1-T804N mScarlet-I-TOSI donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAP_scFv

   Plasmid

   #183733

   Purpose

   Expresses anti-lysozyme scFv antibody

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Anti-lysozyme scFv
   

   Use

   Tags

   His6-tag

   Expression

   Yeast

   Mutation

   Promoter

   AOX1 promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

   Plasmid

   #199454

   Purpose

   U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   TS2 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mouse U6

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA28-pMa-PspCas13b crRNA-TS1

   Plasmid

   #199459

   Purpose

   U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   TS1 crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mouse U6

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRD424

   Plasmid

   #178202

   Purpose

   Expression of Rok from the hns promoter

   Depositor

   Remus Dame

   Article

   Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

   Insert

   phns + rok
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hns promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRD410

   Plasmid

   #178200

   Purpose

   Expression of sRok from the hns promoter

   Depositor

   Remus Dame

   Article

   Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

   Insert

   phns + srok
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hns promoter

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIALD2HMGr

   Plasmid

   #185867

   Purpose

   Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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