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Showing: 3761 - 3780 of 8428 results
  1. pcDNA3-Lyn-dPlcR

    Plasmid
    #181832
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).
    Depositor
    Insert
    Lyn-PlcR
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pME-mKate2-KDEL-pA

    Plasmid
    #109546
    Purpose
    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)
    Depositor
    Insert
    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. LD101: pMVP (L3-L2) eCFP + WPRE

    Plasmid
    #121775
    Purpose
    pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.
    Insert
    eCFP + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid
    #181833
    Purpose
    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.
    Depositor
    Insert
    Lyn-dPlcR (K30L,K32L)
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Lysines 30 and 32 in PH domain mutated to leucine.
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. actc1b-2PH-PLCd-mKate2

    Plasmid
    #109500
    Purpose
    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.
    Depositor
    Insert
    actc1b-2PH-PLCd-mKate2
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3761 - 3780 of 8428 results