actc1b-mKate2-rab24
(Plasmid #109613)

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab24
Depositing Lab

Rob Parton
Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	109613	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pDONR p2R-p3
(Search Vector Database)
Vector type
Zebrafish plasmids

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
rab24
Species
D. rerio (zebrafish)
Entrez Gene
rab24 (a.k.a. im:7142760, im:7155843, zgc:112047)
Tag / Fusion Protein
- mKate2 (N terminal on insert)

Cloning Information

Cloning method Gateway Cloning

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
- Evrogen Limited Use Label License for FPs
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

actc1b-mKate2-rab24 was a gift from Rob Parton (Addgene plasmid # 109613 ; http://n2t.net/addgene:109613 ; RRID:Addgene_109613)
For your References section:

In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG. Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. 10.1038/s41467-020-17486-w PubMed 32709891

actc1b-mKate2-rab24 (Plasmid #109613)