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pGEX-4T-1-Rnt1-dsRBD(+3A)

Plasmid

#171551

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Mutation

a three-alanine insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+6)

Plasmid

#171550

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD

Plasmid

#171549

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (L)

Plasmid

#170624

Purpose

For the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539E

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (L)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-lyn11-myc-LOV2GIVe (D)

Plasmid

#170625

Purpose

For the mammalian expression of mLOV2GIVe dark-mimicking mutation C450S

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (D)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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IBMc120

Plasmid

#161950

Purpose

Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB4A3-PECF20-B30-mScarlet-I-B15

Expression

Bacterial

Promoter

PECF20

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc393

Plasmid

#161953

Purpose

Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB4A3-T-PT7-B30-mScarlet-I-3T

Expression

Bacterial

Promoter

PT7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc330

Plasmid

#161952

Purpose

Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA171-PSrpR-B30-mScarlet-I-Ter

Expression

Bacterial

Promoter

PSrpR

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc050

Plasmid

#161938

Purpose

Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI

Expression

Bacterial

Promoter

Plux2

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc259

Plasmid

#161951

Purpose

Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter

Expression

Bacterial

Promoter

PTet*

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFE-sfGFP

Plasmid

#162688

Purpose

pFE vector control expressing sfGFP

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

sfGFP

Tags

C terminal 6x His tag

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MYMH 391

Plasmid

#138059

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23101- pSEVA-331Bb- RFP

Expression

Bacterial

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 392

Plasmid

#138053

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23102- pSEVA-331Bb- RFP

Expression

Bacterial

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 539

Plasmid

#138078

Purpose

Control vector with no degradation tag to compare against various degradation tag variants

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry::sbfI- pSEVA-331Bb

Expression

Bacterial

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 67

Plasmid

#138048

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-11AT-GFP

Expression

Bacterial

Mutation

AAA GAG GAG ATA

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 49

Plasmid

#138039

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-8AT-GFP

Expression

Bacterial

Mutation

AAA GAG GTG AAA

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH41

Plasmid

#138035

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-7GT-GFP

Expression

Bacterial

Mutation

AAA GAG TAG AAA

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 17

Plasmid

#138023

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-3AC-GFP

Expression

Bacterial

Mutation

AAC GAG GAG AAA

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 553

Plasmid

#138083

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-NYADAS-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 552

Plasmid

#138082

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-DAS- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 551

Plasmid

#138081

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-AAV- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 550

Plasmid

#138080

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-LVA- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 549

Plasmid

#138079

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-LAA- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 473

Plasmid

#138077

Purpose

Synthetic Terminator L3S1P47 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S1P47-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 472

Plasmid

#138076

Purpose

Synthetic Terminator L3S2P44 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S2P44-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 471

Plasmid

#138075

Purpose

Synthetic Terminator L3S2P24 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S2P24-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 470

Plasmid

#138074

Purpose

Synthetic Terminator L3S3P21 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S3P21-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 469

Plasmid

#138073

Purpose

Synthetic Terminator L3S2P21 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S2P21-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 468

Plasmid

#138072

Purpose

Natural Terminator BB0010 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-BB0010-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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