We narrowed to 12,387 results for: cel.2

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pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-NUbo

Plasmid

#212760

Purpose

N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo-MCS

Plasmid

#212725

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His7-NUbo-MCS

Plasmid

#212724

Purpose

Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-MCS-His6

Plasmid

#212723

Purpose

Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSicoR-CENPBi2

Plasmid

#59619

Purpose

Expression of shRNA against human CENPB

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

CENPB (CENPB Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-HA-LMW-FGF2

Plasmid

#157663

Purpose

Expresses FGF2 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deleted amino acid 1-134

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_C942S_pCDNA3.1(+)

Plasmid

#187729

Purpose

Express the catalytically inactive NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_HECT

Plasmid

#187719

Purpose

Express GST-tagged NEDD4L HECT domain in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 594-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-593

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W4-HECT

Plasmid

#187710

Purpose

Express his-tagged NEDD4L W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 543-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-542

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_WW34

Plasmid

#187718

Purpose

Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-581 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

only residues 492-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCD86-mCherry2

Plasmid

#190747

Purpose

Expression mCherry2-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.1

Plasmid

#208300

Purpose

Expresses ABE-RA2.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.1-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, D108G, K110R, H122R, M126I, N127K

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pNCMV 18E6no*

Plasmid

#37456

Purpose

Expression of HPV 18E6no* in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 18E6no*

Tags

Flag and HA

Expression

Mammalian

Mutation

V44A

Promoter

CMV

Available Since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
pHdCas9-mammalian

Plasmid

#138415

Purpose

Expression of Himar-dCas9 in mammalian cells

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pNCMV 5E6

Plasmid

#37459

Purpose

Expression of HPV 5E6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 5E6 (E6 HPV)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pNCMV 6bE6

Plasmid

#37457

Purpose

Expression of HPV 6bE6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 6bE6 (E6 HPV)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pMM95

Plasmid

#176615

Purpose

Extracellular domain truncated. Used to test SIRPA exclusion from the phagocytic synpase.

Depositor

Meghan Morrissey, Ron Vale

Article

Morrissey et al Immunity. 2020 Aug 18;53(2):290-302.e6.

Insert

FRBext-GFPint

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Availability

Academic Institutions and Nonprofits only

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