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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 16,434 results for: jun

Showing: 3761 - 3780 of 16434 results

  3. pfMDH_K381A

    Plasmid
    #67732
    Purpose
    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 381 to alanine
    Promoter
    T7
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pfMDH_N300D

    Plasmid
    #67728
    Purpose
    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to aspartate
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pfMDH_R373A

    Plasmid
    #67731
    Purpose
    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed arginine 373 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pfMDH_N300S

    Plasmid
    #67729
    Purpose
    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to serine
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pAS-7 (mm12-21)

    Plasmid
    #40842
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm12-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pAS-6 (mm17-21)

    Plasmid
    #40841
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm17-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pAS-5 (mm18-21)

    Plasmid
    #40840
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm18-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pAS-4 (mm19-21)

    Plasmid
    #40839
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm19-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pAS-3 (mm20-21)

    Plasmid
    #40838
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm20-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pAS14 (mm16-21)

    Plasmid
    #40849
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm16-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 3761 - 3780 of 16434 results