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CyPet-FKBP-E2

Plasmid

#175244

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81D

Plasmid

#129351

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81D

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu E83A

Plasmid

#129353

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu E83A

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLBS.EF-H2B-QUE37C

Plasmid

#129327

Purpose

ATP measurement, nucleus, lentivirus

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Use

Lentiviral

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pEF1a-rtTAc

Plasmid

#172121

Purpose

Constitutive expression of InteinC-rtTAC

Depositor

Chun Xu

Publication

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

InteinC-rtTAc

Use

AAV

Promoter

EF1a

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-tTAc

Plasmid

#172125

Purpose

Expression of InteinC-tTAC in CaMKII positive cells

Depositor

Chun Xu

Publication

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, tTAc

Use

AAV

Promoter

CaMKII

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

Plasmid

#153195

Purpose

encodes pAAV NovArch

Depositor

Adam Cohen

Publication

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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AL13Rb-HsfA2

Plasmid

#161012

Purpose

PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter

Depositor

Krishna Niyogi

Publication

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-blastR-p2a-v5-dL5

Plasmid

#161980

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Publication

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

blastR-p2a-v5-dL5-GSlinker

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*NoBody

Plasmid

#162807

Purpose

STELLA plasmid for amber suppression of N-terminally labelled NoBody

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)NoBody

Use

Piggybac

Expression

Mammalian

Mutation

NoBody (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pVW293

Plasmid

#139490

Purpose

Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pHSP12 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pHSP12

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_670

Plasmid

#100460

Purpose

Mammalian expression of E. coli LF82_670 CDS fused to mEmerald

Depositor

Alan Huett

Publication

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LF82_670

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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nucHCB2

Plasmid

#98581

Purpose

Nuclear Huntingtin Chromobody 2: "iVHH4" intrabody recognizing amino acids 49–148 of huntingtin, fused to eYFP and nuclear localization signal.

Depositor

Ray Truant

Publication

Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

Insert

HCB2

Tags

NLS and YFP

Expression

Mammalian

Promoter

CMV

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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mKate1.31-CytERM-N-17

Plasmid

#56080

Purpose

Localization: Endoplasmic Reticulum, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Publication

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

CytERM

Tags

mKate1.31 (aka mKate2)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Nano-lantern(ATP0)/pRSETB

Plasmid

#51989

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP0

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
Tom20-mStayGold-NovoSplit657(A)

Plasmid

#255881

Purpose

Expression plasmid for NovoSplit657(A), the first part of the NovoSplit system, fused to the mitochondrial localization signal Tom20 and mStayGold for fluorescence labeling.

Depositor

Julia Mahamid

Publication

Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1

Insert

NovoSplit657(A)

Tags

Tom20 and mStayGold

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM049

Plasmid

#247635

Purpose

to generate mRNA for membrane mStayGold(E138D) for zebrafish

Depositor

Gokul Upadhyayula

Publication

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(E138D)

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-cMyc-Rab3A

Plasmid

#245356

Purpose

Mammalian expression of N-terminal Myc-tagged rat Rab3A wild type

Depositor

Thomas Martin

Insert

Rab3a (Rab3a Rat)

Tags

Myc

Expression

Mammalian

Promoter

EF-1a

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mScarlet-3H (JDW 1513)

Plasmid

#242551

Purpose

Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-H

Use

Gateway Cloning

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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