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Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
UseTagsAviExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7-gfap-sgRNA
Plasmid#65566Purposein vitro trancription of sgRNA targeting the zebrafish gfap locusDepositorInsertgfap sgRNA (gfap Zebrafish)
UseIn vitro rna transcriptionTagsExpressionMutationPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168242Purpose"Rescue Rac expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-WT
UseZebrafish expressionTagsmcherryExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfIl34
Plasmid#168112PurposeExpress zebrafish Il34, mRNA synthesisDepositorInsertIl34 (il34 Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -0
Plasmid#173889PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -1
Plasmid#173890PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-Rpl10a
Plasmid#79880PurposeBeta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
UseTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168243Purpose"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-DN
UseZebrafish expressionTagsmcherryExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168244Purpose"Rescue Rac-CA expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-CA
UseZebrafish expressionTagsmcherryExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
60-p3E_INTRON-EmptyRNAi
Plasmid#163380Purposep3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).DepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG-Mermaid in Tol2
Plasmid#182578PurposeExpresses the FRET membrane voltage sensor Mermaid in vertebrate cellsDepositorInsertCAG-Mermaid in Tol2
UseZebrafish expressionTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Synapse reporters
Plasmid#196899Purposezebrafish expressionDepositorInsertSynapse reporters
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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ZE18 pwnt8 ORF1/2 myc
Plasmid#17052DepositorInsertwnt8 ORF1/2 (wnt8a Zebrafish)
UseTags6xMycExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZP44 Wnt8 5' orf2
Plasmid#16898DepositorInsertwnt8 5'orf2 (wnt8a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
UseTagsEGFPExpressionMutationPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
14UAS zfPSD95:GFP
Plasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi:loxP-EGFP-loxP
Plasmid#27322DepositorInsertubiquitin:loxP-EGFP-loxP
UseMultisite gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only