We narrowed to 1,624 results for: PREP

1. pDONR223_NKX2-1_Long

   Plasmid

   #221471

   Purpose

   Gateway cloning of NKX2-1 long isoform overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_NKX2-1_W208L

   Plasmid

   #221474

   Purpose

   Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   W208L

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_NKX2-1_V205F

   Plasmid

   #221473

   Purpose

   Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   V205F

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX-EF1a_NKX2-1_V205F

   Plasmid

   #221485

   Purpose

   NKX2-1 V205F DNA binding mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   V205F

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX-SFFV_NKX2-1

   Plasmid

   #221496

   Purpose

   NKX2-1 short isoform lentiviral overexpression using SFFV promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1419

   Plasmid

   #219778

   Purpose

   pDEF-TurboID-GFPmut2, negative control for cytosolic expression

   Depositor

   Scott Lindner

   Article

   Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

   Insert

   TurboID/GFPmut2
   

   Use

   Plasmodium, rodent malaria parasites

   Tags

   Expression

   Mutation

   Promoter

   PbEF1alpha promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Efg1-ΔDBD

   Plasmid

   #216396

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 182-356

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Efg1-N-PrLD

   Plasmid

   #216397

   Purpose

   Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-N-PrLD
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 182-554

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-ODN-hsRPL3-CterTwStrep

   Plasmid

   #208644

   Purpose

   for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation

   Tags

   Twin Strep

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM9121_Strep II

    Plasmid

    #153512

    Purpose

    Strep II C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Strep II
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGM9121_TwinStrep

    Plasmid

    #153513

    Purpose

    TwinStrep C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    TwinStrep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGM9121_Aaw-Glucoamylase SP

    Plasmid

    #153497

    Purpose

    Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Glucoamylase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGM9121_Ani- α Amylase SP

    Plasmid

    #153503

    Purpose

    Aspergillus niger α Amylase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    α Amylase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM9121_Sce- α Galactosidase SP

    Plasmid

    #153504

    Purpose

    α Galactosidase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    α Galactosidase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAGM9121_Gga-Lysozym SP

    Plasmid

    #153505

    Purpose

    Chicken Lysozym Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Lysozym SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPAP003

    Plasmid

    #153490

    Purpose

    Level 2 integrative plasmid for stable expression in P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM9121_Sce-Invertase 2 SP

    Plasmid

    #153493

    Purpose

    S. cerevisiae Invertase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Invertase 2 SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJBL7007

    Plasmid

    #136946

    Purpose

    Malachite green aptamer with stability hairpin, bacterial promoter

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

    Insert

    malachite green aptamer (MGA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pInducer20-EGFP-TEADiv2

    Plasmid

    #228876

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP-TEAD inhibitor (TEADiv2)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    TEADiv2
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_dCas9-KRAB-MeCP2_Blast

    Plasmid

    #221501

    Purpose

    Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    dCas9-KRAB-MeCP2-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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