We narrowed to 8,827 results for: Pol

1. pCMV6-AN-DDK_1-590 POLI

   Plasmid

   #131233

   Purpose

   Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a 24 bp insertion within the Pol iota co…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_1-420 POLI

   Plasmid

   #131234

   Purpose

   Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a 24 bp insertion within the Pol iota co…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. IY001: pMVP (L3-L2) BioID2 + polyA

   Plasmid

   #121795

   Purpose

   pMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   BioID2 + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IY202: pMVP (L3-L2) APEX2 + polyA

   Plasmid

   #121796

   Purpose

   pMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   APEX2 + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LC601: pMVP (L3-L2) eYFP + polyA

   Plasmid

   #121769

   Purpose

   pMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eYFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LC701: pMVP (L3-L2) eCFP + polyA

   Plasmid

   #121767

   Purpose

   pMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eCFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7-EF-EYFP-YAP1_R89A-PolyA

   Plasmid

   #112291

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Human YAP1-2gamma with Arginine 89 to Alanine

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7-EF-EYFP-YAP1_L91A-PolyA

   Plasmid

   #112292

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Human YAP1-2gamma with Leucine 91 to Alanine

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7-EF-EYFP-YAP1_F95A-PolyA

   Plasmid

   #112293

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Human YAP1-2gamma with Phenylalanine 95 to Alanine

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL-c5X-poly(alanylglycine)

    Plasmid

    #67003

    Purpose

    Expresses malE-poly(alanylglycine) in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-poly(alanylglycine)
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL-c5X-poly-EAK9

    Plasmid

    #67010

    Purpose

    Expresses malE-poly-EAK9 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-poly-EAK9
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid

    #105648

    Purpose

    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

    Depositor

    Sandra Rieger

    Insert

    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

    Plasmid

    #219826

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA-flag-hKDM4A(H188A)-polyA

    Plasmid

    #101052

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pATetO 6XHis-Taq DNA Pol

    Plasmid

    #178782

    Purpose

    Inducible expression of Taq DNA polymerase in E. coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    Taq DNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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