We narrowed to 9,123 results for: Pol

1. pDest-pol2-Smarcd1-Myc

   Plasmid

   #227716

   Purpose

   Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

   Depositor

   Kent Hunter

   Article

   Ross et al bioRxiv 2024.01.24.577061

   Insert

   Smarcd1 (Smarcd1 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   pol2

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDestTol2pA ins:Apollo-NADP+ mVenus

   Plasmid

   #210518

   Purpose

   Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-HA_aa 1-449 POLH

   Plasmid

   #201583

   Purpose

   Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   POLH (POLH Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-449 of DNA polymerase eta

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-AN-HA_aa 1-542 POLK

   Plasmid

   #201585

   Purpose

   Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   Polymerase kappa (POLK Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-542 of DNA polymerase kap…

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKII-somBiPOLES-ChroME2s-mCerulean

   Plasmid

   #192582

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CaMKII-somBiPOLES-ChroME2f-mCerulean

   Plasmid

   #192581

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T3-GST-PolB(T304I)

   Plasmid

   #177133

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST tol2 UbB polyA

   Plasmid

   #188701

   Purpose

   Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T3-GST-PolB(K72A)

   Plasmid

   #177132

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1C-FlucV5His6-PolyA-HhR_AB

    Plasmid

    #148303

    Purpose

    Insect Expression of PolyA-HhR

    Depositor

    Elisa Izaurralde

    Insert

    PolyA-HhR
    

    Expression

    Insect

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1026M=U6-3-gRNA#2[Pol-γ35]

    Plasmid

    #159675

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#2[Pol-γ35]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC_cherry donor_Polb target (pE038)

    Plasmid

    #149349

    Purpose

    Replace donor vector for cherry insertion using the human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-DDK_RKK/AAA POLI

    Plasmid

    #131240

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert arginine re…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_3KA + S580A POLI

    Plasmid

    #131241

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert Pol iota se…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-DDK_1-590 POLI

    Plasmid

    #131233

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-DDK_1-420 POLI

    Plasmid

    #131234

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. IY001: pMVP (L3-L2) BioID2 + polyA

    Plasmid

    #121795

    Purpose

    pMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    BioID2 + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. IY202: pMVP (L3-L2) APEX2 + polyA

    Plasmid

    #121796

    Purpose

    pMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. LC601: pMVP (L3-L2) eYFP + polyA

    Plasmid

    #121769

    Purpose

    pMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eYFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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