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Showing: 361 - 380 of 593 results

  1. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162687-plasmid-map-sequence-id-321457

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  5. XE25 Dominant Negative Activin Receptor

    Plasmid
    #17005
    Depositor
    Randall Moon
    Insert
    DN Activin Receptor (xstk2 Frog)
    Use
    Xenopus expression
    Tags
    Expression
    Mutation
    Dominant negative
    Promoter
    Available since
    April 15, 2008
    Availability
    Academic Institutions and Nonprofits only

  7. LT3GEPIR-NEK4shRNA1

    Plasmid
    #220544
    Purpose
    To inducibly knockdown NEK4 expression
    Depositor
    Sean Lee
    Insert
    NEK4 shRNA1 (NEK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220544-plasmid-map-sequence-id-438026

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  8. LT3GEPIR-NEK4shRNA2

    Plasmid
    #220545
    Purpose
    To inducibly knockdown NEK4 expression
    Depositor
    Sean Lee
    Insert
    NEK4 shRNA2 (NEK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220545-plasmid-map-sequence-id-438025

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  11. R777-E297 Hs.RPS6KB1

    Plasmid
    #70581
    Purpose
    Gateway ORF clone of human RPS6KB1 [NM_003161.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KB1 (RPS6KB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70581-plasmid-map-sequence-id-126030

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  12. SLK

    Plasmid
    #38963
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLK (SLK Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. R777-E298 Hs.RPS6KB1-nostop

    Plasmid
    #70582
    Purpose
    Gateway ORF clone of human RPS6KB1 [NM_003161.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KB1 (RPS6KB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70582-plasmid-map-sequence-id-126031

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  14. MST4

    Plasmid
    #39172
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MST4 (STK26 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. R777-E299 Hs.RPS6KB2

    Plasmid
    #70583
    Purpose
    Gateway ORF clone of human RPS6KB2 [NM_003952.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KB2 (RPS6KB2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70583-plasmid-map-sequence-id-126032

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  16. R777-E300 Hs.RPS6KB2-nostop

    Plasmid
    #70584
    Purpose
    Gateway ORF clone of human RPS6KB2 [NM_003952.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KB2 (RPS6KB2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70584-plasmid-map-sequence-id-126033

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  18. R777-E091 Hs.FLT3

    Plasmid
    #70375
    Purpose
    Gateway ORF clone of human FLT3 [NM_004119.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FLT3 (FLT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T227M (human SNP)
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70375-plasmid-map-sequence-id-125824

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  19. Lentiviral human Citron shRNA 1 GFP

    Plasmid
    #155286
    Purpose
    Lentiviral expression of human CIT shRNA, GFP expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Citron (CIT Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155286-plasmid-map-sequence-id-310000

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Showing: 361 - 380 of 593 results