We narrowed to 950 results for: cas12a

1. pRDB_031

   Plasmid

   #245331

   Purpose

   Cas12a CRISPRko all-in-one positive control; targets CD47, CD63

   Depositor

   John Doench, David Root

   Insert

   Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

   Plasmid

   #140086

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ220-Ultra

   Plasmid

   #176889

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   AsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE2690

   Plasmid

   #137717

   Purpose

   Expresses dimpLbCas12a-SunTag in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dimpLbCas12a
   

   Use

   CRISPR

   Tags

   NLSx3 SV40 and SunTagx10

   Expression

   Mammalian

   Mutation

   D156R, G532R, K538V, Y542R, K595R, D832A,

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB12A

   Plasmid

   #210035

   Purpose

   Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas12a
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE3646

   Plasmid

   #137714

   Purpose

   Expresses LbCas12a RVRR variant in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   LbCas12a-RVRR
   

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE3987

   Plasmid

   #137716

   Purpose

   Expresses dLbCas12a-RVRR-BE in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dLbCas12a-RVRR-BE
   

   Tags

   6xHis, APOBEC-1, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   G532A, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ10333

   Plasmid

   #183955

   Purpose

   CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   Nuclease-dead Lb Cas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC64420

    Plasmid

    #209517

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC70364

    Plasmid

    #209515

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC67844

    Plasmid

    #209514

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EC70202

    Plasmid

    #209463

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    TaU6

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EC67907

    Plasmid

    #211794

    Purpose

    Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    HptII, Cas9, GR-GRF-GIF
    

    Expression

    Plant

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC70190

    Plasmid

    #209451

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC70205

    Plasmid

    #209466

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    TaU6

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. EC70196

    Plasmid

    #209457

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    TaU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFD8

    Plasmid

    #140619

    Purpose

    Expression of one of several LbCas12a crRNAs in Drosophila

    Depositor

    Michael Boutros

    Article

    Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Drosophila U6:3

    Available Since

    Oct. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC70188

    Plasmid

    #209449

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EC70189

    Plasmid

    #209450

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge