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pQHA-USP7 CS puroR

Plasmid

#46754

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-NLS-BirA-2a-mCherry

Plasmid

#79886

Purpose

Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Ubiquitin

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 H-RAS

Plasmid

#236449

Purpose

transient overexpression of H-RAS in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

H-RAS (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pUG-125

Plasmid

#104636

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_EL4

Use

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

AvrPtoB-Phe479Ala

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-413

Plasmid

#104639

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_Myc-Fen_EL5

Use

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

AvrPtoB-Phe479Ala

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pER8 cHA-FG1

Plasmid

#118689

Purpose

Plant inducible expression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AT3G07200 (AT3G07200 Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

LexA

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pER8 cHA-AIP2

Plasmid

#118687

Purpose

Plant inducible expression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AIP2 (AT5G20910 Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

LexA

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pER8 cHA-XERICO

Plasmid

#118690

Purpose

Plant inducible expression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

LexA

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBA cHA-FG1

Plasmid

#118665

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AT3G07200 (AT3G07200 Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBA cHA-XERICO

Plasmid

#118666

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK5 wt

Plasmid

#80876

Purpose

mammalian expression of ALK5 WT

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only

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V51 pIRESpuro-GLUE (pGLUE)

Plasmid

#15100

Purpose

tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK5 T204D

Plasmid

#80877

Purpose

mammalian expression of constitutively active ALK5 T204D

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T204D (constitutively active)

Promoter

CMV

Available since

Aug. 26, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-ALK5 K232R

Plasmid

#80878

Purpose

mammalian expression of ALK5 K232R

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K232R (kinase dead)

Promoter

CMV

Available since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFS217-USP44_MSCV-TAP

Plasmid

#43896

Purpose

Retroviral expression of USP44 in mammalian cells

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Retroviral

Tags

FLAG, HA, and HIS

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2013

Availability

Academic Institutions and Nonprofits only
pCINeo CD3 delta

Plasmid

#201814

Purpose

Mammalian Expression

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

CD3-delta (Cd3d Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU1

Plasmid

#133978

Purpose

mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-RNF168 delta MIU2

Plasmid

#133979

Purpose

mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU2

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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M76 pIRESpuro-GLUE-hDsh2

Plasmid

#15101

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

Dsh2 (DVL2 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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M77 pIRESpuro-GLUE-hDsh3

Plasmid

#15102

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

Dsh3 (DVL3 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge