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EKv407(Gold Cas-guide insert3)

Plasmid

#248336

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv406(Gold Cas-guide insert2)

Plasmid

#248335

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv410(Gold Cas-guide insert6)

Plasmid

#248339

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv411(Gold Cas-guide insert7)

Plasmid

#248340

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv412(Gold Cas-guide insert8)

Plasmid

#248341

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv405(Gold Cas-guide insert1)

Plasmid

#248334

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv409(Gold Cas-guide insert5)

Plasmid

#248338

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-U6-BRD4-HaloTag-Guide-hspCas9

Plasmid

#228576

Purpose

Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

Depositor

Marcin Imielinski

Insert

BRD4 gRNA (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 1

Plasmid

#198489

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide

Plasmid

#121993

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide#2

Plasmid

#121994

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-opa1 guide

Plasmid

#121995

Purpose

An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

opa1 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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LOC5574680 Atrial natriuretic peptide receptor

Plasmid

#115374

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5574680 Atrial natriuretic peptide receptor (LOC5574680 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod2 guide

Plasmid

#107604

Purpose

An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod1 guide

Plasmid

#107603

Purpose

An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-uqcrc1 guide

Plasmid

#107602

Purpose

An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

uqcrc1 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)(mature peptide)-pcw107-V5

Plasmid

#64643

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Mutation

G23V

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)(mature peptide)-pcw107

Plasmid

#64642

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

Plasmid

#246438

Purpose

All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.

Depositor

Ailong Ke

Article

Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

Inserts

NmeCas9
Nme-sgRNA

Tags

HA tag, NLS, and SV40 NLS

Expression

Mammalian

Mutation

changed Aspartic acid 16 to Alanine, deleted amin…

Promoter

EF-1 alpha and U6

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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