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pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

Plasmid

#74302

Purpose

Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

Depositor

Ali Guler

Publication

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)

Use

Zebrafish

Tags

FLAG, cmcl2::EGFP, and p2A-mCherry

Mutation

TRPV4: delta 760-871

Promoter

Neurog1

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-MAGT1

Plasmid

#132302

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MAGT1 (MAGT1 Human)

Expression

Mammalian

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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8X2C mAGNET

Plasmid

#67955

Purpose

Lentiviral vector coding EGFP followed by 8x miR-128 and 8x miR-221 target sequences

Depositor

Xue Han, Wilson Wong

Publication

Sayeg et al ACS Synth Biol. 2015 Apr 13.

Type

Empty backbone

Use

Lentiviral

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Publication

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGLO-Magenta-AZA

Plasmid

#220784

Purpose

Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

Depositor

Angela Leu

Insert

magenta chromoprotein

Tags

6xHis

Expression

Bacterial

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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MAGE-A3Pro pGL4basic

Plasmid

#78138

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Publication

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAGE-A3 (MAGEA3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -418/+12 of promoter

Promoter

MAGE-A3

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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PRS415-Gal1pr-Mag1-sctetR

Plasmid

#44752

Depositor

Narendra Maheshri

Publication

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

MAG1-sctetR

Expression

Yeast

Promoter

Gal1pr

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Plasmid

#176310

Purpose

Expresses CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Publication

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

CreN59_nMag_NLS_P2A_mCherry_IRES_puroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

human EF1alpha

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pORTMAGE302A

Plasmid

#128970

Purpose

Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction

Depositor

George Church

Inserts

PapRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Promoter

cI-pL

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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MAGI1_PDZ_2

Plasmid

#103899

Purpose

Protein expression and purification of MAGI1 PDZ2 domain

Depositor

Sachdev Sidhu

Publication

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSCALPS-mAgo2-EGFP

Plasmid

#209935

Purpose

Lentiviral expression of mouse Argonaute-2 in mammalian cells

Depositor

Phillip Zamore

Publication

Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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GST-MAGE-2-429

Plasmid

#89129

Purpose

bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

Depositor

Elizabeth Wilson

Publication

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
MAGE-A12Pro pGL4basic

Plasmid

#78139

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Publication

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAGE-A12 (MAGEA12 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -425/+115 of promoter

Promoter

MAGE-A12

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-SomaGCaMP7

Plasmid

#182941

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsMago_I

Plasmid

#146579

Purpose

Mammalian Expression of HsMago

Depositor

Elisa Izaurralde

Publication

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HsMago (MAGOH Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Publication

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CMV

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Publication

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Expression

Mammalian

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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dClover2-N1

Plasmid

#54538

Purpose

Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pNCS-Antares

Plasmid

#74279

Purpose

Constitutive expression of Antares in bacteria

Depositor

Michael Lin

Publication

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Antares

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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mTFP1-pBAD

Plasmid

#54553

Purpose

Localization: Protein Expression Vector , Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Publication

Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

Type

Empty backbone

Tags

6xHis and mTFP1

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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