We narrowed to 8,304 results for: construct

1. pSKB3-SaGGR-I206F-L377H (JBEI-7202)

   Plasmid

   #110152

   Purpose

   pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

   Depositor

   Jay Keasling

   Article

   Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

   Insert

   GGR
   

   Use

   Tags

   TEV-cleavable His-tag

   Expression

   Bacterial

   Mutation

   SaGGR-I206F-L377H mutant gene

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGC5941-PacI-ath-STTM865-5p

   Plasmid

   #84186

   Purpose

   target miRNA865-5p for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM865-5p
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCGN N-Ras 12D, 189L

   Plasmid

   #14726

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   N-ras (NRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   12D, 189L

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

4. MTK1_018

   Plasmid

   #123681

   Purpose

   Encodes ConL8 as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFGC5941-PacI-ath-STTM863-3p

   Plasmid

   #84182

   Purpose

   target miRNA863-3p for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM863-3p
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFGC5941-PacI-ath-STTM863-5p

   Plasmid

   #84183

   Purpose

   target miRNA863-5p for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM863-5p
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MTK5_011

   Plasmid

   #123855

   Purpose

   Encodes ConR8 as a Type 5 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector R8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK2_014

   Plasmid

   #123706

   Purpose

   Encodes pUAS (miniCMV core) as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pUAS (mini CMV)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTK4_004

   Plasmid

   #123813

   Purpose

   Encodes spacer sequence as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Spacer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTK2_018

    Plasmid

    #123710

    Purpose

    Encodes truncated pCMV as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pCMV-C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-TAL-NC2

    Plasmid

    #43856

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    Flag and FokI nuclease

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFGC5941-PacI-ath-STTM319/159

    Plasmid

    #84116

    Purpose

    target miRNA319/159 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM319/159
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMaSat-miRFP670 pc3944

    Plasmid

    #201695

    Purpose

    Expresses of miRFP-tagged MaSat in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    MaSat Synthetic construct zinc finger
    

    Use

    Tags

    miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA

    Plasmid

    #222404

    Purpose

    arabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.

    Depositor

    Katherine Berry

    Article

    Linh D. Nguyen et al Sci Rep 15, 3820 (2025)

    Insert

    5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
    

    Use

    Tags

    1xMS2 hairpin, 7GC clamp, TtrpA terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. GL4.10 Prg4 promoter-luciferase

    Plasmid

    #195034

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter (PRG4 Bovine)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

    Plasmid

    #195037

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTC3cIfm

    Plasmid

    #193778

    Purpose

    Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Frame-shifted CI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO-1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196081

    Purpose

    Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196083

    Purpose

    Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196075

    Purpose

    Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge