We narrowed to 2,461 results for: organization Addgene

1. SOX9-T2A-H2B-EGFP repair template

   Plasmid

   #167972

   Purpose

   SOX9 reporter repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 9 (SOX9 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

   Plasmid

   #127355

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TPC1-mCherry

   Plasmid

   #135182

   Purpose

   Expresses TPC1 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC1 (Tpcn1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

   Plasmid

   #127369

   Purpose

   Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Plant

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204188

   Purpose

   Doxycycline inducible lentiviral vector for human NOTUM overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NOTUM (NOTUM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LAMP1-mCherry-FRB*

   Plasmid

   #207145

   Purpose

   Lysosome-targeted rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   FRB* (T2098L mutant of FKBP-rapamycin binding dom…

   Expression

   Mammalian

   Mutation

   Silent mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

   Plasmid

   #176395

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

   Plasmid

   #127356

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mito-mTurq2-Apollo-NADP+

   Plasmid

   #193303

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   mTurqoise2 and mitochondrial targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cyto-mTurq2-Apollo-NADP+

    Plasmid

    #193301

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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