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pKG-GW1-GlcisPT

Plasmid

#203172

Purpose

Yeast expression vector for Giardia cis-prenyltransferase

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

UPPS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hSynapsin-Nluc-EPAC-VV

Plasmid

#204846

Purpose

Produce lentivirus encoding Nluc-EPAC sensor in neuronal cell type

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

Nluc-EPAC sensor

Use

Lentiviral

Available Since

Aug. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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LENTICRISPR-SNRPA_sgRNA1

Plasmid

#201622

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA2

Plasmid

#201623

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-HA-BioID2-SAPAP3

Plasmid

#176858

Purpose

Expresses SAPAP3 protein fused with BioID2 at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-SAPAP3

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_1-140_AG

Plasmid

#148832

Purpose

Bacterial Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBam_1-140_AG

Plasmid

#148815

Purpose

Insect Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam_1-140_AG

Plasmid

#148820

Purpose

Bacterial Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-GGSG-DmBam_13-36_AG

Plasmid

#148827

Purpose

Insect Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-DmCaf40_25-291-V186E_AF

Plasmid

#148735

Purpose

Bacterial Expression of DmCaf40_25-291-V186E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40_25-291-V186E (Rcd-1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6_AF

Plasmid

#148738

Purpose

Bacterial Expression of HsNot6

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot6 (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmNot1_1468-1719_AF

Plasmid

#148740

Purpose

Bacterial Expression of DmNot1_1468-1719

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmNot1_1468-1719 (Not1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-AAGAB

Plasmid

#198332

Purpose

Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-DmCaf40_25-291-Y139DG146W_AF

Plasmid

#148736

Purpose

Bacterial Expression of DmCaf40_25-291-Y139DG146W

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40_25-291-Y139DG146W (Rcd-1 Fly)

Expression

Bacterial

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Chinchilla–insM

Plasmid

#191226

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed methionine at 122 in the protein sequence

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Chinchilla+N122D–insM

Plasmid

#191227

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed methionine at 122 and replaced asparagine…

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Sandgrouse–insD

Plasmid

#191228

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed aspartic acid at 121 in the protein seque…

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG

Plasmid

#148841

Purpose

Mammalian Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KEE +E111H

Plasmid

#187431

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed glutamic acid at 117 to histidine

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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