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pWZL Neo Myr Flag RPS6KA2

Plasmid

#20621

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KA2 (RPS6KA2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pWZL Neo Myr Flag SNF1LK

Plasmid

#20629

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

SNF1LK (SIK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TTK

Plasmid

#20659

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TTK (TTK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-BEACON2

Plasmid

#171698

Purpose

Expresses BEACON2 in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

Insert

BEACON2

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_W98Y/W104A/Y130F

Promoter

CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FUW-TetO-Gata6

Plasmid

#128827

Purpose

dox-dependent expression of mouse Gata6

Depositor

Yossi Buganim

Publication

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Publication

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT3TS-Cre

Plasmid

#47117

Purpose

Can be used to in vitro transcribe Cre recombinase.

Depositor

Stephen Ekker

Publication

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

Cre

Promoter

Lac

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Publication

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pVL1393-MAL-SYK

Plasmid

#157990

Purpose

Expresses SYK in insect cell (sf9)

Depositor

Byung Il Lee

Publication

Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

Insert

Spleen tyrosine kinase (SYK Human)

Tags

Met-Ala-Leu and add Leu,Glu residues +His tag

Expression

Insect

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dnmt3b-R

Plasmid

#122333

Purpose

Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dnmt3b

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dnmt3b-L

Plasmid

#122334

Purpose

Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dnmt3b

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-EF-Slc2a3-L

Plasmid

#122304

Purpose

Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119067

Purpose

Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Expression

Bacterial

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pANY3

Plasmid

#112197

Purpose

This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

Depositor

Yingfeng An

Publication

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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33-G-X1-pEF1-puro

Plasmid

#111604

Purpose

Express human 33-G-X1 in 293T cells

Depositor

Timothy Springer

Publication

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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33-G-X5-pEF1/V5-His

Plasmid

#111605

Purpose

Express human 33-G-X5 in 293T cells

Depositor

Timothy Springer

Publication

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCaSpeR3/ncd-gfp #10

Plasmid

#66082

Purpose

stable germline transformation of Drosophila to produce Ncd-GFP expression

Depositor

Sharyn Endow

Publication

Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

Insert

Ncd kinesin-14 motor protein (ncd Fly)

Tags

GFP

Expression

Insect

Mutation

D699L

Promoter

ncd promoter (3.1kb upstream region of ncd start …

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pBEST-melR-deGFP-T500

Plasmid

#45396

Depositor

Vincent Noireaux

Publication

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

melibiose repressor

Expression

Bacterial

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBADmod1-linker-deGFP

Plasmid

#45397

Depositor

Vincent Noireaux

Publication

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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