We narrowed to 31,033 results for: uros

1. XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

   Plasmid

   #233435

   Purpose

   PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

   Plasmid

   #233426

   Purpose

   PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

   Plasmid

   #228400

   Purpose

   Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   NES/PKI-mNeonGreen2x-NLS/FUS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

   Plasmid

   #228398

   Purpose

   Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   NLS/cMyc-AcGFP2x-NES/iKB2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

   Plasmid

   #218916

   Purpose

   Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

   Plasmid

   #216871

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

   Insert

   Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   mdx mutation in mouse dystrophin

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

   Plasmid

   #179434

   Purpose

   Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-HS4-mch (SH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174118

   Purpose

   Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid

    #183410

    Purpose

    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    KRAB-dCas9-ecDHFR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid

    #171384

    Purpose

    Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized dCas13X.1-RESCUE-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

    Plasmid

    #140086

    Purpose

    Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    LbCpf1 (LbCas12a) crRNA backbone
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid

    #171385

    Purpose

    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized minidCas13X.1-RESCUE-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid

    #209080

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

    Plasmid

    #86230

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    AID[71-114]-eGFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-MTS-G3635A-L17-FZY2-S100N-T26I&T77I-UGI-Puro

    Plasmid

    #209645

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100N-T26I&T77I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179436

    Purpose

    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    HS4-TRE-cit-HS4-mch (DH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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