We narrowed to 88,655 results for: Mal;

1. mutpRL-TK 1x perfect to bulged siRNA

   Plasmid

   #40765

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to bulged target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. mutpRL-TK 1x perfect to seed siRNA

   Plasmid

   #40766

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to seed target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

3. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

   Plasmid

   #40767

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to seed plus 13-16 target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

4. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_FOXA2_P2A_Hygro_Barcode

   Plasmid

   #120439

   Purpose

   Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   FOXA2 (FOXA2 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K63

   Plasmid

   #17606

   Purpose

   Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K63 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-Ubiquitin-K48

   Plasmid

   #17605

   Purpose

   Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-Cre

   Plasmid

   #198712

   Purpose

   For lentiviral expression of Cre recombinase in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Insert

   Pgk-Cre
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xFlag-tdMCP-Erk2 (pErk reporter)

   Plasmid

   #226167

   Purpose

   Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   3xFlag-tdMCP-Erk2 (MAPK1 Human)
   

   Tags

   3xFlag-tdMCP

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA6 asyn WT

    Plasmid

    #107425

    Purpose

    Mammalian expression of human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein (SNCA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Mfn2-HA

    Plasmid

    #139192

    Purpose

    Expresses C-terminal HA tagged wt human Mfn2 in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Tags

    3HA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH0006

    Plasmid

    #135448

    Purpose

    Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    dCas9-BFP-KRAB
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6 asyn E46K

    Plasmid

    #105730

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Expression

    Mammalian

    Mutation

    E46K

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-hMC4R

    Plasmid

    #179315

    Purpose

    Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    melanocortin 4 receptor (MC4R Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF CAG luc hygro

    Plasmid

    #67502

    Purpose

    Constitutive expression of firefly luciferase in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRPML1-G-GECO1.2-ERES

    Plasmid

    #207144

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Mcoln1 (MCOLN1 Human)
    

    Tags

    ER-Export Signal (ERES) and G-GECO1.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_KLF17_P2A_Hygro_Barcode

    Plasmid

    #120503

    Purpose

    Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF17 (KLF17 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. RpH-ILV-3xALFA

    Plasmid

    #225135

    Purpose

    RpH-ILV probe strongly colocalises with lysosomal markers.

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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