We narrowed to 4,220 results for: PRS

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pV1382

Plasmid

#111436

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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p306-BrdU-Inc

Plasmid

#71792

Purpose

BrdU-incorporating plasmid with selectable marker URA3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mCherry

Plasmid

#226567

Purpose

Encodes siRNA resistant SigmaR1 protein labeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCSN075

Plasmid

#166732

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pV1326

Plasmid

#111435

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSBK.079

Plasmid

#187218

Purpose

Expresses Eco1 RT and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 Reverse Transcriptase
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23115 and T7/lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

Plasmid

#89693

Purpose

Luciferase reporter plasmid targeted at csr-1 locus

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gsy-1 promoter, luciferase

Use

Yeast episomal vector

Tags

luciferase

Promoter

gsy-1

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDuRCC-K

Plasmid

#81193

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below., Please view…

Promoter

ROX3 and SNP52p

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen R119A

Plasmid

#226572

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

R119A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen D188A

Plasmid

#226573

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

D188A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen F191A

Plasmid

#226571

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

F191A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNCS tdTomato

Plasmid

#91767

Purpose

Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

tandem dimer Tomato

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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p415-PtrpC-Cas9-TtrpC-CYC1t

Plasmid

#68059

Purpose

Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

humanized Cas9

Use

CRISPR; N. crassa, fungi

Expression

Yeast

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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(189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

Plasmid

#160739

Purpose

Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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p405-BrdU-Inc

Plasmid

#71791

Purpose

BrdU-incorporating plasmid with selectable marker LEU2

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-PEX3

Plasmid

#51572

Purpose

Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

PEX3 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pSLS.405

Plasmid

#187203

Purpose

Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA (wildtype)
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPMVAd3

Plasmid

#98298

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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