We narrowed to 9,007 results for: mel

1. MMMMA-pHG165c

   Plasmid

   #170108

   Purpose

   Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

   Depositor

   Liskin Swint-Kruse

   Article

   Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

   Insert

   MelR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM1160c2

   Plasmid

   #159724

   Purpose

   Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Use

   Tags

   Gal4DBD and MPGIL-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM1674

   Plasmid

   #159726

   Purpose

   Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Use

   Tags

   Gal4DBD and MPTLV-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM1479a3

   Plasmid

   #159725

   Purpose

   Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Use

   Tags

   Gal4DBD and MYITV-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCKan-yibDp-CBD-hGLY

   Plasmid

   #134596

   Purpose

   Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   human glycine acyltransferase 2 (GLYATL2 )
   

   Use

   Tags

   chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-T2-GpNLuc

   Plasmid

   #135949

   Purpose

   Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   GpNLuc LumiFluor
   

   Use

   Tags

   T2 mitochondrial localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a-T2-OgNLuc

   Plasmid

   #135951

   Purpose

   Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   OgNLuc LumiFluor
   

   Use

   Tags

   T2 mitochondrial localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSTAP-seq_fly-ssp3

   Plasmid

   #86382

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster ssp3 enhancer
   

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSTAP-seq_fly-ham

   Plasmid

   #86383

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster ham enhancer
   

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSTAP-seq_fly-sgl

    Plasmid

    #86384

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster sgl enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSTAP-seq_fly-ncm

    Plasmid

    #86385

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ncm enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMART-Ala1

    Plasmid

    #65814

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Insulated waaHp from E. coli

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Use

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-pAG-Hia5

    Plasmid

    #174373

    Purpose

    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein AG - hia5
    

    Use

    Tags

    6xHis and Protein A/G

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe_Zeo_cOVA_T2A_mStrawberry

    Plasmid

    #161736

    Purpose

    Expression of cOVA and mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    cytoplasmic ovalbumin
    

    Use

    Retroviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe_Hyg_cOVA_T2A_mStrawberry

    Plasmid

    #161737

    Purpose

    Expression of cOVA and mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    cytoplasmic ovalbumin
    

    Use

    Retroviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MiniCoopR U6:gRNA spred1, mitfa:Cas9

    Plasmid

    #118843

    Purpose

    Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    spred1 gRNA (spred1 Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. delCMV-mCherry-2x-Rhotekin-GBD

    Plasmid

    #214142

    Purpose

    Rho activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xRhotekin-GBD (Rtkn Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLAG AXL

    Plasmid

    #105933

    Purpose

    Mammalian expression of Flag-tagged AXL

    Depositor

    Rosa Marina Melillo

    Article

    Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

    Insert

    AXL (AXL Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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