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Dead Streptavidin-SpyCatcher
Plasmid#59547PurposepET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramersDepositorInsertDead-dloop-Streptavidin-SpyCatcher
UseTagsN-terminally shortened SpyCatcherExpressionBacterialMutationPolyAsp in L3/4 loop for ion-exchangePromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Nedd2-like caspase (DRONC)
Plasmid#157778PurposeE. coli expression vector pET23 with C-term 6xHis tagDepositorInsertDrosophila Nedd2-like caspase (DRONC) (Dronc Fly)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc LbaCas12a
Plasmid#182123PurposepET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP5
Plasmid#128939PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, ZDepositorInsertsUseTagsHisExpressionBacterialMutationResidues 234-241 are substituted for a PreScissio…PromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc AspCas12a
Plasmid#182120PurposepET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc LbaCas12a
Plasmid#182125PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc enAspCas12a
Plasmid#182127PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc enAspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only
