We narrowed to 16,982 results for: bon
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Plasmid#140201PurposeFungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.DepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Cloning of crrna anā¦TagsdLbCas12a crRNA scaffoldExpressionMutationPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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pEGFP-C3-PLK4-S305E-3xFLAG
Plasmid#69840PurposeThis plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationS305EPromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-GFP
Plasmid#82506PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-S285A/T289A-3xFLAG
Plasmid#69841PurposeThis plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationS285A/T289APromoterCMVAvailable sinceDec. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS2044
Plasmid#79652PurposessAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple255-EmGFP WPRE
UseAAVTagsExpressionMutationPromoterPAX6 HS234Z EnhancerAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
Plasmid#160616PurposeTU for the constitutive expression of Streptomyces phage PhiC31 integrase.DepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-FLAG-NEMO-C54/347A
Plasmid#27268DepositorInsertNEMO (IKBKG Human)
UseTagsFLAGExpressionMammalianMutationCys54Ala Cys347AlaPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS2049
Plasmid#79657PurposessAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple260-EmGFP WPRE
UseAAVTagsExpressionMutationPromoterPAX6 Retinal EnhancerAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2045
Plasmid#79653PurposessAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple256-EmGFP WPRE
UseAAVTagsExpressionMutationPromoterPAX6 enhancerAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2048
Plasmid#79656PurposessAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple259-EmGFP WPRE
UseAAVTagsExpressionMutationPromoterPAX6 MiniPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-llo-mc38tmg
Plasmid#174603Purposeexpresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1DepositorInsertextracell transmem domains moCD19 wCterm fusion LLO190 and minigenes of 3neoantigens MC38tumor in genes ADGPK DPAGT Reps1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2043
Plasmid#79651PurposessAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple254-EmGFP WPRE
UseAAVTagsExpressionMutationPromoterPAX6 Retinal EnhancerAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARB365
Plasmid#124048PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB9
Plasmid#63110PurposeExpression of HSPB9 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb9 (HSPB9 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
NG1301 pCI-TPI-4MS2-SMG5(short)-4H
Plasmid#65806PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4 MS2 stem loops, SMG5 3'UTR shortened versiā¦ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-KLF9 3'UTR
Plasmid#84599PurposeRenilla luciferase reporter containing KLF9 3'UTRDepositorInsertKLF9 3'UTR (KLF9 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS1925
Plasmid#105869PurposeHigh copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmidDepositorInsertiCreERT2
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only