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pBad33_ISAba30_reRNA

Plasmid

#205323

Purpose

Bacterial expression of ISAba30 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAba30 TnpB

Expression

Bacterial

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRS426-ACT_stable

Plasmid

#127609

Purpose

high-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Stable ACT1 intron (Mut)

Expression

Yeast

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CoA-Snifit(G41)

Plasmid

#197945

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-mIFP-NLS

Plasmid

#173893

Purpose

for mIFP expression in nucleus

Depositor

Ron Vale

Article

Yan et al J Cell Biol. 2021 Feb 1;220(2). pii: 211

Insert

mIFP

Use

Lentiviral

Promoter

SSFV

Available Since

Nov. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAA41

Plasmid

#128281

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, parS (3.2 kb spacer between parS and Piq)

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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HDHD3

Plasmid

#39029

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

HDHD3 (HDHD3 Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
T7-WbpB-His6

Plasmid

#89711

Purpose

Expresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

WbpB (wbpB Pseudomonas aeruginosa)

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20

Plasmid

#243747

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_Δpvc10

Plasmid

#243742

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc12-FLAG_Δpvc10

Plasmid

#243738

Purpose

PVC structural operon - FLAG-tagged baseplate (Pvc12) - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc12-FLAG_ Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-AvrPWT4

Plasmid

#237516

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Plant

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTA7001-AvrPWT4-GFP

Plasmid

#237532

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Plant

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTA7001-RWT4Ca_noKDup

Plasmid

#237531

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_noKDup

Expression

Plant

Mutation

Remove KDup region

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTA7001-VirPWT4-GFP

Plasmid

#237533

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Plant

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-VirPWT4

Plasmid

#237517

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca

Plasmid

#237509

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca_Reg1

Plasmid

#237525

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Yeast

Mutation

Removed binding region 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Cs

Plasmid

#237510

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Cs

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:GST-AvrPWT4

Plasmid

#237522

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-RWT4Ca

Plasmid

#237519

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTA7001-RWT4Ca_KDup

Plasmid

#237534

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Dup

Expression

Plant

Mutation

Only contains KDup region

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-RWT4Ca

Plasmid

#237518

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:GST-VirPWT4

Plasmid

#237523

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-RWT4Ca_noKDup

Plasmid

#237535

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_noKDup

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0041 Ac5-FRB-MCP

Plasmid

#233412

Purpose

AC5 promoter driven FRB-MCP for drosophila cell expression

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FRB-MCP

Expression

Mammalian

Mutation

WT

Promoter

Ac5

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0030 SFFV-bArrestin2-MCP (FLP-IN)

Plasmid

#233436

Purpose

SFFV driven ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM052 CMV-TO-CCR6-PCP (FLP-IN)

Plasmid

#233406

Purpose

CCR6-V2-PCP with a 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-PCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ024 CMV-TO-bArrestin2-MCP (FLP-IN)

Plasmid

#233379

Purpose

ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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MOBE2

Plasmid

#219942

Purpose

Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-3'end_PT2A_apt-ABE8.20

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0194 CMVTO-secNLuc-rens-mem

Plasmid

#231211

Purpose

Membrane-bound nanoluciferase

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

secNLuc-rens-mem

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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