Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,039 results for: SIM

Showing: 5881 - 5910 of 7039 results

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pTwist Amp High Copy FAM136A-HA WT

Plasmid

#244883

Purpose

In vitro transcription of wild-type FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy ENDOG-HA

Plasmid

#244881

Purpose

In vitro transcription of human ENDOG downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ENDOG (ENDOG Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-FAM136A-mutant-3xFLAG-hygro

Plasmid

#244879

Purpose

Overexpression of mutant human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

GRCh38, chr2:70300842G>A

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg2

Plasmid

#244864

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg1

Plasmid

#244863

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg2

Plasmid

#244852

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR IMMP2L sg1

Plasmid

#244851

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874)

Plasmid

#239353

Purpose

Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(WT)-mascRNA(mut 8356-8370) reporter.

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA(mut 8356-8370)

Expression

Mammalian

Mutation

MALAT1 ENE(WT)-mascRNA(mut 8356-8370: ctacgaccacc…

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_RPP21 (pAVA3259)

Plasmid

#239327

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPP21

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPP21 (RPP21 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_BRF2 (pAVA3258)

Plasmid

#239326

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting BRF2

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting BRF2 (BRF2 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TCTN3 minigene (pAVA3939)

Plasmid

#239345

Purpose

Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG)

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG-BGH polyA (TCTN3 Human)

Tags

3xFLAG, 6xMyc, and CopGFP

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/MALAT1[C8351G] (pAVA3169)

Plasmid

#239346

Purpose

Expresses full-length, driven by its own promoter MALAT1 with stability-compromised ENE(C8351G) and an 11-nt insertion (cgctcgacgta).

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

10,843 bp of MALAT1 of genomic locus amplified from genomic DNA of Hela cells (MALAT1 Human)

Expression

Mammalian

Mutation

An 11-nt insertion (cgctcgacgta) and stability-co…

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC6 (pP18(T)_B11-AVA4069)

Plasmid

#239304

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC6

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC6 (EXOSC6 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_EXOSC7 (pP18(T)_C4-AVA4066)

Plasmid

#239305

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC7

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC7 (EXOSC7 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_EXOSC8 (pP18(T)_B1-AVA4071)

Plasmid

#239306

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC8

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC8 (EXOSC8 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_EXOSC9 (pP18(T)_A7-AVA4072)

Plasmid

#239307

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC9

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC9 (EXOSC9 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_DIS3 (pP18(T)_A5-AVA4073)

Plasmid

#239308

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DIS3

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DIS3 (DIS3 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_2xsgRNAs_RBM7 (pAVA3250)

Plasmid

#239322

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RBM7

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RBM7 (RBM7 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_2xsgRNAs_SKIV2L2 (pAVA3251)

Plasmid

#239323

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting SKIV2L2

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting SKIV2L2 (MTREX Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_2xsgRNAs_ZCRB1 (pAVA3300)

Plasmid

#239325

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNA stargeting ZCRB1

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting ZCRB1 (ZCRB1 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_EXOSC10 (pAVA3318)

Plasmid

#239295

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC10

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC10 (EXOSC10 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070)

Plasmid

#239296

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting C1D (C1D Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_EXOSC2 (pP18(T)_C3-AVA4067)

Plasmid

#239301

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC2

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC2 (EXOSC2 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065)

Plasmid

#239302

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC4 (EXOSC4 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA_EXOSC5 (pP18(T)_B12-AVA4068)

Plasmid

#239303

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC5

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC5 (EXOSC5 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPc1 PTEN C124S

Plasmid

#237340

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

C124S

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDUET-1_VNp-anti-Muc1 mAb

Plasmid

#214954

Purpose

Bacterial expression of anti-Muc1 (human) mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-Muc1 mAb heavy chain (MUC1 )
VNp-anti-Muc1 mAb light chain (MUC1 )

Tags

6xHis and VNp

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S91, 262 ,331, 724A

Plasmid

#224738

Purpose

For expression of HA-tagged GTSE1 protein with S91, 262 ,331, 724A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 91, 262 ,331, 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP

Plasmid

#223214

Purpose

Lentiviral vector - mCherry reporter and sTNFaR for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

Soluble TNFαR (TNFRSF1A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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