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PB-PGK-FKBP-Zte-Cit-hc-DHFR

Plasmid

#116047

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Zte-Cit-hc-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-FKBP-Zte-Cit-hc-DHFR

Plasmid

#116059

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Zte-Cit-hc-DHFR

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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NK640

Plasmid

#109103

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK642

Plasmid

#109101

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK591

Plasmid

#109080

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mTFP1

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1331 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]

Plasmid

#107854

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

W385G in splicing reporter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only
NK613

Plasmid

#109091

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry_SV40 NLS

Use

Choanoflagellate expression

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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NK648

Plasmid

#109104

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

3'UTR EFL

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK597

Plasmid

#109086

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

tdTomato

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK595

Plasmid

#109083

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

EGFP

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK585

Plasmid

#109079

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK609

Plasmid

#109078

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK588

Plasmid

#109077

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

pActAvrII_nanoluc

Use

Choanoflagellate expression

Mutation

Eliminate NcoI site in the promoter

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK606

Plasmid

#109076

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK587

Plasmid

#109075

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1337 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309S]

Plasmid

#107849

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G309S in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

Plasmid

#107847

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All asparagine to glutamine in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

Plasmid

#107843

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All phenylalanine to serine in splicing reporter

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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p5lysAsfGFP

Plasmid

#92025

Purpose

sfGFP reporter plasmid with lysA TALE binding site in its promoter

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Insert

sfGFP

Use

TALEN

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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p5sucAmCherry

Plasmid

#92026

Purpose

mCherry reporter plasmid with sucA TALE binding site in its promoter

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Insert

mCherry

Use

TALEN

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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