We narrowed to 10,106 results for: HA
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GreenT-EC - Display - CyRFP1
Plasmid#193943PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTHD422N
Plasmid#194432PurposeAPOBEC1 fused to YTHD422NDepositorInsertAPOBEC1-YTHD422N (APOBEC1 Human)
UseCRISPRTagsAPOBEC1-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…PromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN HAYFP-LOV24
Plasmid#49570Purposeregulate protein stability with lightDepositorInsertYFP-LOV24
UseRetroviralTagsHA and YFPExpressionMutationdelete the last 3 aa from AsLOV2, with I532A muta…PromoterLTRAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV LDTS-eLIR
Plasmid#189000Purposelipophagy-inducing adaptor proteinDepositorInsertLDTS-eLIR
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 T194D
Plasmid#80886Purposemammalian expression of ALK7 T194DDepositorInsertALK7 (Acvr1c Rat)
UseTagsHAExpressionMammalianMutationT194D (constitutively active)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
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GreenT-EC.b - Display - CyRFP1
Plasmid#193944PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC.b
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt1
Plasmid#49186PurposecDNA of Akt1 with myristyolation signal in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCsC_spacer
Plasmid#136077PurposeUse when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assignedDepositorInsertEven spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCsE_spacer
Plasmid#136078PurposeUse when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assignedDepositorInsertEven spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
GreenT-EC.c - Display - CyRFP1
Plasmid#193945PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC.c
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 K222R
Plasmid#80887Purposemammalian expression of ALK7 K222RDepositorInsertALK7 (Acvr1c Rat)
UseTagsHAExpressionMammalianMutationK222RPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z18-PDGFR_EF1a_eGFP
Plasmid#186304PurposePlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ18-PDGFR
eGFP
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FRBl2-E
Plasmid#15289DepositorInsertFRAP-rapamycin binding domain (MTOR Human)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationres. 2025-2113, T2098LPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG mCherry-LDTS-eLIR
Plasmid#189002Purposelipophagy-inducing adaptor protein, AAVDepositorInsertLDTS-eLIR, mCherry
UseAAVTagsHAExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorInsertsI-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-I-SceI-Trex2
Plasmid#44024DepositorInsertI-SceI fused to mTREX2
UseTagsNLS-HAExpressionMammalianMutationPromoterpCAGGSAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
V51 pIRESpuro-GLUE (pGLUE)
Plasmid#15100Purposetandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tagsDepositorTypeEmpty backboneUseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2+_HA-mAID-nanobody (Kless)
Plasmid#117718PurposemRNA synthesis of lysine-less HA-mAID-nanobdy for injectionDepositorInsertmAID-nanobody
UseTags3xHAExpressionMammalianMutationcodon-optimized and lysine-less mAID for murine e…PromoterSP6Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TKGAPDH in pLJM60
Plasmid#154217PurposeLentiviral vector for stable expression of FLAG-TK-GAPDHDepositorInsertGAPDH
UseLentiviralTagsFLAGExpressionMammalianMutationcDNA has been codon optimized for human cell expr…PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM201
Plasmid#173179PurposedCas9-24xGCN4DepositorInsertdCas9-24xGCN4
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM122: pAAV-EFS-CasRx
Plasmid#166870PurposeAAV vector for expressing CasRxDepositorInsertRfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationPromoterEFSAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-3XHA
Plasmid#127881PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA taggingDepositorInsertGFP-Puro-3XHA (PPP2CA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP
Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC - GPI
Plasmid#193946PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC
UseTagsGPI-anchor and Igk_secretion - HA-FlagExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS158
Plasmid#215679PurposeEmpty repair plasmid for ChrIII split hygromycinR landing padDepositorInsert5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt(K179M)
Plasmid#39535DepositorInsertAkt(K179M) (Akt1 Mouse)
UseTags6G linker, EGFP, and HAExpressionMammalianMutationK179MPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_tdMS2cp-tagRFP-Tx2
Plasmid#119908PurposeePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusionDepositorInsertHA-tdMS2cp-tagRFP-Tx2-nls
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
E.coli RaPID plasmid
Plasmid#107251PurposeExpresses the E.coli RaPID labeling proteinDepositorInsertE.coli BirA*
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
p11d-sctRPA
Plasmid#102614PurposeExpression of S. cerevisiea RPA in E.coliDepositorUseTagsExpressionBacterialMutationRFA2 has intron deletedPromoterT7Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.S738E/S742E
Plasmid#10810DepositorInsertPKD (PRKD1 Human)
UseTagsHAExpressionMammalianMutationActivation loop active: S738E and S742E (bp 2212-…PromoterCMVAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.K/W
Plasmid#10809DepositorInsertPKD (PRKD1 Human)
UseTagsHA and Myc (inserted between amino acids 33&3…ExpressionMammalianMutationKinase dead mutant: K612W (Lys 612 to Trp) (bp183…PromoterCMVAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW
Plasmid#133359PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNoTag-DGCR8
Plasmid#164694PurposeExpress DGCR8 without tag in mammalian cellsDepositorInsertDGCR8 (DGCR8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only
