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pDONR221-HUWE1_UBM-mut

Plasmid

#187149

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

L2976A/P2977A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA-mut

Plasmid

#187145

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

M1328A/F1330A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_BH3-4E

Plasmid

#187143

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

V1976E/V1780E/L1983E/M1987E

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta169-189

Plasmid

#187134

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta169-189

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3896-3951

Plasmid

#187132

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta3896-3951

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_I3969A/F3982A

Plasmid

#187128

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

I3969A/F3982A

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_YH/GG_delta3291-3298

Plasmid

#187126

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

Y355G/H356G, delta3291-3298

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3291-3298

Plasmid

#187124

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta3291-3298

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_YH/GG

Plasmid

#187122

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

Y355G/H356G

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-BTLA (PD-1 ITIM)-mGFP

Plasmid

#180818

Purpose

Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

BTLA (PD-1 ITIM) (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Mutation

replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

Promoter

SFFV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#2

Plasmid

#163389

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#1

Plasmid

#163388

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#3

Plasmid

#163390

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ash1-5p

Plasmid

#169712

Purpose

Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

Depositor

Tanja Mittag

Article

Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

Insert

Ash1 (ASH1 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

Truncation including encoding amino acids 420-480…

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFM038

Plasmid

#40141

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

R183-5A + 7SA; base pairs 6-523 of par-2 were re…

Promoter

pie-1

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM041

Plasmid

#40144

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (S241A) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM039

Plasmid

#40142

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

R183-5A + S241A; base pairs 6-523 of par-2 were …

Promoter

pie-1

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM036

Plasmid

#40139

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (K162A) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00646

Plasmid

#39708

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1705 (Met Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only