We narrowed to 4,214 results for: Abo;

1. pQE60 P123G wgMDH

   Plasmid

   #204256

   Purpose

   Bacterial expression of P123G wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   P123G

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE60 P123F wgMDH

   Plasmid

   #204255

   Purpose

   Bacterial expression of P123F wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   P123F

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

   Plasmid

   #200389

   Purpose

   3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   PX458_3XHA_dFnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. JAB3479

   Plasmid

   #190417

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. JAB3278

   Plasmid

   #190402

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. JAB3616

   Plasmid

   #190426

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. JAB2601

   Plasmid

   #190388

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. JAB2600

   Plasmid

   #190387

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. JAB2599

   Plasmid

   #190386

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXD70Tet(DadH)-Pdadh-DadhABC(A2)

    Plasmid

    #191644

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid

    #168770

    Purpose

    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

    Plasmid

    #141452

    Purpose

    B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Cdk6 (Cdk6 Mouse)
    

    Use

    Mouse Targeting; Dre / rox

    Tags

    C-terminal fusion of T2A-TagRFPT-dre-PBD*

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128347

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    control (negative)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pRSETC PITPbeta N89F

    Plasmid

    #58279

    Purpose

    bacterial expression of PITP beta N89F mutant

    Depositor

    Shamshad Cockcroft

    Article

    Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N89F (abolishes PI binding)

    Promoter

    T7

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

20. MP9956:SFG.GD3synthase-2A-GD2synthase

    Plasmid

    #75013

    Purpose

    Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

    Depositor

    Martin Pule

    Article

    Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

    Inserts

    murine GD3 synthase
    murine GD2 synthase
    

    Use

    Retroviral; Momlv retroviral vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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