We narrowed to 4,014 results for: Gast

1. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

   Plasmid

   #172920

   Purpose

   LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7s-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

   Plasmid

   #172919

   Purpose

   LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7f-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

   Plasmid

   #172914

   Purpose

   Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7c-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

   Plasmid

   #172913

   Purpose

   Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7b-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAct-Cas9

   Plasmid

   #62209

   Purpose

   Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pnos-Cas9-nos

   Plasmid

   #62208

   Purpose

   Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJAT62

   Plasmid

   #204301

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJAT56

   Plasmid

   #204300

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PRExpress-Bxb1-hsp70pA

   Plasmid

   #158391

   Purpose

   heat shock inducible Bxb1 integrase for drosophila melanogaster

   Depositor

   Michael Elowitz

   Article

   Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

   Insert

   Bxb1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UAS-Ceru-10unit

    Plasmid

    #158389

    Purpose

    10-unit intMEMOIR recording array for Drosophila melanogaster

    Depositor

    Michael Elowitz

    Article

    Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

    Insert

    10unit recording array
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUASt-ssBiP-ESCargo

    Plasmid

    #140166

    Purpose

    Expresses a regulatable secretory cargo for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hs70

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. act5C-mScarlet

    Plasmid

    #230906

    Purpose

    Ubiquitous expression of mScarlet in Drosophila melanogaster

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJAT17

    Plasmid

    #204298

    Purpose

    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLib6.6

    Plasmid

    #176652

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLib6.4B

    Plasmid

    #176665

    Purpose

    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLib6.6B

    Plasmid

    #176666

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEM-WingGFP-tan

    Plasmid

    #83473

    Purpose

    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    WingGFP-tan
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Hsp70 and enhancer from D. melanogaster yellow ge…

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUASt-ssBiP-ESCargo*

    Plasmid

    #140165

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hs70

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster U6:3

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RP78 pUAST Orco mRNA

    Plasmid

    #53431

    Purpose

    Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    Orco (Orco )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only